Appendix 6. Figures - randd.defra.gov.ukrandd.defra.gov.uk ›...
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Appendix 6. Figures Coefficient 0.29 0.46 0.63 0.80 0.98 ShaheenBasmati Azucena Jasmati KDML105 MahiSugandha IR64 KalingaIII Sugandha-1 Sherbati1 Sherbati2 PusaSugandha1 PusaSugandha2 PusaSugandha3 Pak386 Supra Basmati198 Basmati370 Ranbir Taraori SuperBasmati Basmati2000 ShaheenBasmati Basmati385F Pusa1121 PusaBasmati Figure 1A. 24 rice varieties with 41 InDel markers. Coefficient 0.27 0.45 0.64 0.82 1.00 HaryanaBasmati Basmati370 DehraDun Basmati217 Ranbir Taraori Kernel Basmati198 Yamini SuperBasmati PunjabBasmati Basmati2000 ShaheenBasmati Basmati385 Azucena Jasmati PusaBasmati Pusa1121 PusaSugandha2 PusaSugandha3 MahiSugandha KDML105 MugadSugandha Sugandha-1 Superfine PusaSugandha1 Sherbati1 Sherbati(awns) IR64 KalingaIII Sherbati2 Supra Pak386 Kasturi HaryanaBasmati Figure 1B. 34 rice varieties with a sub-set of nine InDel markers
Transcript of Appendix 6. Figures - randd.defra.gov.ukrandd.defra.gov.uk ›...
Appendix 6. Figures
Coefficient0.29 0.46 0.63 0.80 0.98
ShaheenBasmati
Azucena Jasmati KDML105 MahiSugandha IR64 KalingaIII Sugandha-1 Sherbati1 Sherbati2 PusaSugandha1 PusaSugandha2 PusaSugandha3 Pak386 Supra Basmati198 Basmati370 Ranbir Taraori SuperBasmati Basmati2000 ShaheenBasmati Basmati385F Pusa1121 PusaBasmati
Figure 1A. 24 rice varieties with 41 InDel markers.
Coefficient0.27 0.45 0.64 0.82 1.00
HaryanaBasmati
Basmati370 DehraDun Basmati217 Ranbir Taraori Kernel Basmati198 Yamini SuperBasmati PunjabBasmati Basmati2000 ShaheenBasmati Basmati385 Azucena Jasmati PusaBasmati Pusa1121 PusaSugandha2 PusaSugandha3 MahiSugandha KDML105 MugadSugandha Sugandha-1 Superfine PusaSugandha1 Sherbati1 Sherbati(awns) IR64 KalingaIII Sherbati2 Supra Pak386 Kasturi HaryanaBasmati
Figure 1B. 34 rice varieties with a sub-set of nine InDel markers
Figure 2. Example of ISSR fragments separated on the Bioanalyser. The problem of variable band intensity meant that this marker system was not considered further.
Pusasugandha
II
Pusasugandha
I
Supra
Pak 386
SherbatiA
SherbatiII
SherbatiI
ShaheenB
as
Basm
ati 2000
Super
PusaB
asmati 1
Taraori
Figure 3.. INDEL Multiplex panel 1 (IMPX1).
Figure 4a. Four INDEL variety test
Figure 4b. Four INDEL variety test
Figure 4a. Four INDEL variety test
Figure 5. Agilent fingerprint profiles for 12 different samples tested with 4-INDEL test. (Note ignore the band between 400 and 500 bp in lane 4; this is an artifact of the software).
Figure 6. Manual Height Threshold (FU) indicated by a green line is reduced from 20 to 2 which allows the area for the 168 non-basmati peak to be given.
Figure 7a. INDEL B8 Quantitative test showing replicate test samples
Figure 7. b. INDEL B8 quantitative test showing standards
Quantitative SOP Validation
0.07.0
14.021.028.035.042.049.056.063.070.077.084.0
CEQ Agilent Agilent Agilent Agilent
RM201 SOP1(RM201)
SOP1(RM201)
SOP2(INDEL)
SOP2(INDEL)
FDNAS FDNAS WSS FDNAS WSS
Estim
ated
% n
on-b
asm
ati
2861
3021
3028
3101
Figure 8 Validation of Quantitative SOPs in two laboratories (WSS and FDNAS). The results for four different commercial “unknown”samples are shown relative to Quantification with RM201 on the CEQ 8000 (CEQ). Note the permitted level of non-basmati is 7%.
