UMR CNRS 6022 Génie Enzymatique et Cellulaire (Directeur : A. Friboulet) Thème 1 : Biocatalyse et...
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Transcript of UMR CNRS 6022 Génie Enzymatique et Cellulaire (Directeur : A. Friboulet) Thème 1 : Biocatalyse et...
UMR CNRS 6022Génie Enzymatique et Cellulaire
(Directeur : A. Friboulet)
Thème 1 : Biocatalyse et Alternatives Métaboliques (Prof. D. Thomas)
Thème 2 : Biocatalyse supramoléculaire et anisotrope
•Métabolisme lipidique végétal (Dr. B. Thomasset, Dr. Y. Perrin)
•Génèse et caractérisation de la diversité biocatalytique (Dr. A. Friboulet, Dr. B. Bihan-Avalle, Dr. S. Padiolleau, Dr C. Bedel)
•Polymères biomimétiques, impression moléculaire et nanostructuration (Pr. K. Haupt, Dr A. Cordin)
•RMN des solides et systèmes biomimétiques (Pr. C. Sarazin)
•Modèles biomimétiques membranaires (Pr. S. Pulvin, Dr. S. Morandat, Dr. V. Ducel, Dr. C. Rossi)
Affinity separation
Biosensors Biochips Immunoanalysis
Directed synthesis
and catalysis
AntibodiesEnzymes
Hormon receptors
Highly specific molecular recognition
but
UnstableExpensive
Sometimes difficult to obtain
SYNTHETIC RECEPTORS ?
Drug targeting
Antigen
Monomers Complex Imprinted polymer
MOLECULARLY IMPRINTED POLYMERS -SYNTHETIC ANTIBODIES
CHARACTERISTICS
Tailor-made materials
Physical Stability
Chemical Stability
Storage Endurance
Imprint Memory
Binding Strength
Capacity
Easy integration in industrialfabrication processes
Resistant against mechanical stress, highpressures and temperatures up to 120ºC
Resistant against acids, bases, variousorganic solvents, metal ions
Years at RT without loss of performance
Repeated use without loss of memory
KD in nM - µM range
100 µg/g … 10 mg/g
APPLICATIONS
Antibody / receptor binding site mimics
Immunoassays
Drug development / screening
Sensors
Chemical and Biosensors
Multisensors and Biochips
Tailor-made separation materials
Facilitated synthesis / Catalysis (enzyme mimics)
Slow release matrices
Removal of unwanted molecules
Biomedical, agriculture, food, textile,environment, security…
OUR ACTIVITIES
Material engineering: Morphology (Films, nanoparticles…)Water-compatibilityRecognition properties
Surface engineering: NanostructuresPorosityWetting propertiesOptical properties
Separation: Selective extractionChromatography
Sensitive and selective Biomimetic assaysdetection: Acoustic and optical sensors
BiochipsMEMS
BIOMIMETICS
QuickTime™ and aTIFF (LZW) decompressor
are needed to see this picture.
ELISA-TYPE IMMUNOASSAY
MIPnanospheres
Conjugate2,4-D
Advantages• No laboratory animals !• Fast and easy• Sensitive• High throughput• Robotisable
Chemiluminescence(H2O2, luminol)
N NH
Tobaccoperoxidase
Detection:
2,4-DO C
Cl
Cl O
Antigen-enzyme conjugate
H
Competitor, g/ml
B/B0, %
20
40
60
80
100
0.01 0.1 1 10 100 1000
1
2
3
4
Competitor
1: 2,4-Dichlorophenoxyacetic acid (2,4-D)2: 4-Chlorophenoxyacetic acid3: Phenoxyacetic acid4: 2,4-D-methylester
X-reactivit
y
100 %10 %2 %<1 %
O COOH
Cl
Cl
2,4-D(Template)
1 2 3 4
Competitorconcentration
96-well plate
384-well plate
BIOPUCES
Deposition of monomer solution with microcantilever array
Imprinted polymer (model system) Trimethylolpropane trimethacrylate 4-Vinylpyridine Porogen : DMSO/Diglyme Antigen : Fluorescein
Photopolymerisation (DPAP, 360 nm)
Vandevelde et al. (2007) Langmuir 23, 6490-6493.
NANOSTRUCTURED SURFACES BY NANOMOULDING
CHANGE OF SURFACE PROPERTIES
Flat surface
Nanostructuredsurface
0 min 1 min 3 min
• Poly(divinylbenzene-co-methacrylic acid) MIP• Environmental scanning electron microscopy• Condensation of water
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
Water dropleton lotus leaf
• SAS since 19 July 2004, Rouen, Normandy, France
• Founders : Kaynoush Naraghi Sami Bayoudh Karsten Haupt
• Developing innovative polymers : Molecularly imprinted polymers, responsive hydrogels, associative polymers
• Life and pharmaceutical sciences, food industry, agroindustry
• In 2004, winner of the Contest "Innovative and Technological Companies" of the French Ministry of Research&Technology
• Currently 10 people
• 3rd round of fund raising beginning 2008
VALORISATION