Développement et distribution de

Serial Cloner

15 mai 2012, Journée PLUME Biologie, Montpellier

Franck Perez, CNRS UMR144

Institut CurieFranck.Perez@curie.fr

• Licence, Maitrise, DEA de Génétique

• Thèse de Biologie Cellulaire

• 3 ans à l’Université de Génève

• Recruté par le CNRS en 1999 (Biologie)

• Directeur de Recherche CNRS en 2003

• Chef d’équipe à l’Institut Curie et Co-responsable d’une plateforme de criblage et d’une plateforme d’anticorps recombinants

Court CV

• Amstrad : ! ! Locomotive BASIC! ! ! ! Assembleur Z80

• PC :! ! ! ! TURBO PASCAL

• AtariST! ! ...

• MacIntosh! ! ...

Expérience / Programmation

• Hobbiste, autodidacte

Programmer un Macintosh

“Découverte” de RealBasic V2.x / V3.x

• BASIC orienté objet

• Génère un exécutable

• Plug-in en C/C++ si besoin

• Apparence “native” des applications produites

• Temps de développement “relativement” court

• Nombreux exemples, Web sites, forums

Aujourd’hui nommé “Real Studio”

Utilisation pour développer des utilitaires pour le laboratoire

MT-Simul Dimitrov et al., 2008

Développement de petits logiciels pour le labo

Image Extractor

Multi-HitList Compare Folders

RealStudio

PO BOX #162181Austin TX, 78716

voice: 512-328-7325fax: 512-328-7372

http://www.realsoftware.com/

Which edition is right for you?

Feature Personal Professional Enterprise Web

Best for: Hobbyists and Students Part-time developers Full-time developers All developers

Compiles native, high-quality applications

Development platforms Mac OS X, Windowsor Linux

Mac OS X, Windowsand Linux

Mac OS X, Windows,Linux and the web

the web

Networking support for TCP, UDP, Web (HTTP), Email(POP3 and SMTP) and SOAP

Playing Movies

Extensive support for bitmap and vector graphics

Support for Apple events, Keychain, Spotlight, the Dockand the Address Book (Mac Only) *

Includes RealSQLDatabase for single user applications

Use and decrypt encrypted classes

Report Editor

Encrypting classes

ServerSocket Class (More Info)

SSL Support (More Info)

Create console/cgi/service applications

Database server connectivity (Oracle, ODBC, Postgre,MySQL, Microsoft SQL [Windows only])

AES 128 Database Encryption

Remote Debugging (More Info)

ContainerControls (More Info)

Auto Discovery (More Info)

Technical support

Deploy on Mac OS X, Windows, Linux

Develop on Mac OS X, Windows and Linux

Deploy on OS X, Windows & Linux web servers

Profiler for code optimization (More Info)

IDE Scripting

Build Automation (More Info)

Priority technical support

# of updates included with new license 6 months 6 months 12 months 6 months

New License Price 79,00€ 249,00€ 825,00€ 475,00€

# of updates included with renewal 12 months 12 months 12 months 12 months

12 Month Renewal Price 39,00€ 120,00€ 415,00€ 239,00€

• Developpemement rapide

• interface native, MacOS, Windows, Linux

• Executable relativement véloce

MAIS

• Licence payante

• Communauté réduite

Serial Cloner

Biologie moléculaire / Génie génétique

Biologie moléculaire / Génie génétique

Le modèle : DNA Strider

• Très puissant (écrit en C)• Très utilisé• “simple”• Peu cher (400 € / Labo)

• interface vieillissante• peu ergonomique• très “textuel”• pas interactif• pas d’aide au clonage• pas de version Windows

Biologie moléculaire / Génie génétique

Développement de Serial Cloner

• Développé depuis 2005 (OS9)• Distribué depuis 2007• “Développeur/utilisateur”• Gratuit (donationware)

• simplicité• interface réactive, graphique• connection sources externes• aide au clonage• Version Mac & Windows (+Linux)

Serial Cloner

• Version Windows

• Version MacOSX

Serial Cloner - Demo

Serial Cloner - Demo

Serial Cloner - Demo

http://www.serialbasics.com

Home Softwares Serial Cloner Serial List MT Simul Serial Dialer Serial Cleaner

Laboratory tools

Distribution

Serial Cloner (Distribution Web)Home Softwares Serial Cloner Serial List MT Simul Serial Dialer Serial Cleaner

Serial Cloner is a Molecular Biology software.

It provides tools with an intuitive interfacethat assists you in DNA cloning, sequenceanalysis and visualization.

Serial Cloner is freeware and is available forMacOSX , Windows and Linux.

Serial Cloner also lets youbuild text restriction map andquickly format it to add multi-frame translation or onlyshow single cutters forexample. Since version 2.1,Sequence Features arevisible in the Sequence Map.The graphic map of SerialCloner is really Graphic asyou can easily select andextract a fragment or showsingle, double or multiplecutter all in the samewindow. All the features aredisplayed on the map usinguser-modifiable colors; the features can be manually entered, importedfrom GenBank or automatically found after scanning by Serial Cloner. The

All the tools you need to analyze and manipulate your sequences areavailable in an all-in-one-window concept. Numerically select fragments,find restriction sites, ORF or any nucleotide or peptide sequence, calculateTm of selected fragments, %GC or dynamically determine the translationyour selection into peptide and calculate the MW using a compactinterface.

Main Functions

Don’t clone alone

Fully Graphical MapSequence Window

Serial Cloner has been developed to provide a light yet powerful molecularbiology software to both Macintosh and Windows users. A Linux version isalso developped. Serial Cloner reads and write DNA Strider-compatiblefiles and import and export files in the universal FASTA format. SerialCloner also import files saved in the Vector NTI, MacVector, ApE,DNAstar, pDRAW32 and GenBank formats. Import from VectorNTI multi-file format is also possible now. Powerful graphical display tools andsimple interfaces help the analysis and construction steps in a veryintuitive way. Serial Cloner 2.5, handles Annotations and Features both inthe sequence and in the Graphic Map and can automatically scan forsequence Features.

Overview

An intuitive Interface

Sequence Alignment Sequence Map

Multi-Format importScan for Features

pDRAW32, ApE,Fasta, GenBank,MacVector,Vector NTI, DNAStrider, Text

Home Softwares Serial Cloner Serial List MT Simul Serial Dialer Serial Cleaner

Serial Cloner

SerialCloner 2-5 Installer

This version is provided “as is” and is unsupported. It has onlybeen tested on Ubuntu and has known interface problems butseems quite usable in 2.5, although not at the level of theother platform versions

version_2-5-details.txt

Serial Cloner is a Molecular Biology software.

It provides tools with an intuitive interface that assistsyou in DNA cloning, sequence analysis andvisualization.

Serial Cloner is freewareand is available for MacOSX, Windows and Linux

To Install Serial Cloner (Mac and Windows):Either download the installer or the compressed archive on the Version you want to install. When using the Installer, simply run it and follow theinstruction. When using the archive, download the file (Disk image for MacOSX or compressed Zipped file For Window, un-compress it and copythe resulting Serial Cloner Folder in your Application folder. Erase previous versions of Serial Cloner.For Linux, download the compressed tar archive, uncompress and copy Serial Cloner Folder in your Application folder.

Don’t clone alone

SerialCloner 2-5 Zip File

SerialCloner 2-5 Installer

SerialCloner 2-5 Disk Image

SerialCloner2-5 tar Archive

SerialCloner 2-5 (Universal) Installer

Serial Cloner 2-5 (Universal) disk image

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100 000 téléchargements sur 5 ans (5 versions)

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Serial Cloner - Utilisateurs

Serial Cloner - Licence

• Distribution comme freeware (Gratuiciel) / donationware• via un site web dédié

• Le code source n’est pas disponible• plus de 90% de utilisateurs ne sont pas des développeurs• code source peu professionnel, peu commenté• développement seul, jamais développé en groupe• contrôle des objectifs du logiciel, de l’interface• concept d’utilisateur - développeur

• Evolution du logiciel (suggestion/correction)• Utilisation personnelle• Equipe, amis & collègues• Email• Forum