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10

References1. Anonymous. 2012. Statistical repertoire of Iranian Tobacco Company. Behshahr:

Iranian Tobacco Company Publishing. 52p. (In Persian).2. Campbell C L and Madden L V. 1990. Introduction to plant Disease Epidemiology.

London: John Wiley & Sons, Inc. 532 p.3. Campbell C L, Jacobi W R, Powell N T and Main C E. 1984. Analysis of disease

progression and the randomness of occurrence of infected plants during tobaccoblack shank epidemics. Phytopathology 74: 230–235.

4. Cardoso J E, Santos A A, Rossetti A G and Vidal J C. 2004. Relationship betweenincidence and severity of cashew gummosis in semiarid north-eastern Brazil. PlantPathology 53: 363–367.

5. Elahinia A. 1998. Mycology and plant pathology. Rasht: University of GuilanPublishing. 533 p. (In Persian).

6. Ershad J, Zalpour N and Makki M. 1974. Occurrence of black shank disease oftobacco in Iran. Iranian Journal of Plant Pathology 10 (1): 92–100.

7. Ferrin D M and Mitchell D J. 1986. Influence of initial density and distribution ofinoculum on the epidemiology of tobacco black shank. Phytopathology 76: 1153–1158.

8. Ferrin D M and Mitchell D J. 1986. Influence of soil water status on theepidemiology of tobacco black shank. Phytopathology 76: 1213–1217.

9. Guan J and Nutter FWJ. 2003. Quantifying the interarater repeatability andinterrater reliability of visual and remote-sensing disease-assessment methods in thealfalfa foliar pathosystem. Canadian Journal of Plant Pathology 25: 143–149.

10. Jacobi W R, Main C E and Powell N T. 1983. Influence of temperature and rainfallon the development of tobacco black shank. Phytopathology 73: 139–143.

11. James W C. 1971. An illustrated series of assessment keys for plant disease, theirpreparation and usage. Canadian Plant Disease Survey 51: 39–65.

12. Johnson A W, Csinos A S, Golden A M and Glaze N C. 1992. Chemigation forcontrol of black shank-root-knot complex and weeds in tobacco. Supplement toJournal of Nematology 24(4S): 648–655.

13. Kannwischer M E and Mitchell D J. 1978. The influence of a fungicide on theepidemiology of black shank of tobacco. Phytopathology 68: 1760–1765.

14. Lucas G B. 1975. Disease of Tobacco, 3rd edition. Releight: Biological consultingAssociates. 621pp.

15. Najjafi M, Salavati M, Sajjadi A and Afshari Azad H. 2013. Efficacy of somefungicides against tobacco damping off caused by soilborne pathogens. Paperpresented at: 21th Iranian Plant Protection Congress; 23-26 August; Urmia, Iran.

16. Powell N T and Nusbaum R. 1960. The black shank root knot complex in fluecured tobacco. Phytopathology 50: 899–906

17. Sajjadi A and Assemi H. 2012.Antifungal activity of plant extracts of catmint,tobacco and thyme on fungal pathogens of tobacco. Biological Control of Pests andPlant Diseases 3(1): 41–52.

18. Sajjadi A and Assemi H. 2012.Identification of pathogenic soilborne fungi oftobacco in Golestan province fields. Applied Plant Protection 1(3): 233–248.

19. Sajjadi A, Hosseininejad A and Assemi H. 2014. Identification and physiologicalraces of root-knot nematode species (Meloidogyne spp.) in the tobacco fields inGolestan province, Iran. Applied Plant Protection 1(3): 233–248.

/ / /139611

20. Shengfu Y, Shew H D and Barker K R. 1994. International of Meloidogyneincognita, Phytophthora nicotianae var nicotianae and Metalaxyl on resistant andsusceptible tobacco. Journal of Nematology 26(4): 538–571.

21. Shew H D. 1987. Effect of host resistance on spread of Phytophthora parasiticavar. nicotianae and subsequent development of tobacco black shank under fieldconditions. Phytopathology 77: 1090–1093.

22. Van Jaarsveld E, Wingfield M J and Drenth A. 2003. A Rapid seedling basedscreening technique to assay tobacco for resistance to Phytophthora nicotianae.Journal of Phytopathology 151: 389–394.

12

Research in Plant Pathology/Vol. 5/No. 2/Autumn and Winter 2017-20181

Status of tobacco black shank disease in Golestan province

A. Sajjadi*1, M.A. Aghajani2, H. Assemi1, M.R. Najafi1

AbstractThe casual agent of tobacco black shank disease, Phytophthora nicotianae Breda de Haan

(=P. parasitica Dasture), is one of the most important pathogen in tobacco fields. Disease canoccur at all stages of plant growth and damage can occasionally reach up to 100% in somefields. In order to determine the status of the disease in tobacco fields of Golestan province,45 tobacco fields were selected in five different village of Gorgan regions (Taghartappeh,Jafarabad, Ghorogh, Nodehmalek and Valeshabad) and in four different village of Aliabadregions (Pichakmahaleh, Baraftan, Fazelabad and Elazman) during 2014 growth season. Thedisease amount on aerial parts of tobacco plants was recorded during the infection periodaccording to weekly surveys in the selected fields started when the primary symptoms becomeapparent. Statistical analysis was performed using the Stat Graphics Centurion XV software.The disease incidence was similar in different regions but there was significant difference(P 0.01) among the disease incidence of the fields of a village. The highest disease incidence(34.4%) was observed in a field located in Valeshabad village. The highest (22.96%) andlowest (13.72%) disease incidence was recorded in Valeshabad village and Nodehmalekvillage, respectively. There was no statistical difference among the areas under diseaseprogress curves in different regions, however significant difference (P 0.01) was observedamong the areas under disease progress curves of the fields of a village. The maximum areaunder disease progress curve (1743.3) was recorded in a filed in Ghorogh village. Themaximum (1146) and minimum (641.76) area under disease progress curve was calculated forBaraftan village and Nodehmalek village, respectively. The results of this study showed thattobacco black shank has spread extensively in the farms of Golestan province with variousdisease incidences.

Keywords: Area under disease progress curves, disease incidence, infection period,Phytophthora nicotianae.

1- Research Instructor, Department of Plant Protection, Tirtash Research and Education Center, Behshahr,Iran.

2- Assistant Professor, Agricultural and Natural Resources, Research Center of Golestan, Gorgan, Iran.*Corresponding author: Sajjadi_a@yahoo.com