Download - Biotechniques Participant Copy

BIOTECHNIQUES

Mr. Thaddeus Owen D. Ayuste Faculty of Science, Technology and

Mathematics

College of Teacher Development Philippine Normal University

1

1. A teacher will show actual feeding habits of some animals to his students. Which will be the best visual aid?

A. Microtechniques

B. Taxidermy

C. Herbarium

D. Aquarium

2

From Part II: Analyzing Test Items-Question # 4

1. A teacher will show actual feeding habits of some animals to his students. Which will be the best visual aid?

3

Micro techniques

Taxidermy

Herbarium

Aquarium


2. Luis is ready to cut specimens thinly for slide preparation. What instrument does he need?

A. Stain

B. Incubator

C. Microtome

D. Fine needles

4


2. Luis is ready to cut specimens thinly for slide preparation. What instrument does he need?

5

Sectioning

Stain

Incubator

Microtome

Fine needles


3. If an incubator is to be improvised, which will provide the source of heat?

A. Bulb

B. Lightning

C. Porcelain

D. Thermometer

6


3. If an incubator is to be improvised, which will provide the source of heat?

7

Bulb

Lightning

Porcelain

Thermo- meter


4. A teacher will use herbarium in teaching. Which topic is suitable for herbarium?

A. Identifying the name of the plant

B. Osmosis of water to leaves

C. Showing photosynthesis

D. Respiration in plants

8


4. A teacher will use herbarium in teaching. Which topic is suitable for herbarium?

9

Preserved plants; dead plants

Identifying the name of the plant

Osmosis of water to leaves

Showing photosynthesis

Respiration in plants


5. A teacher will use taxidermy in teaching. Which topic is suitable for taxidermy?

A. Skin covering

B. Feeding habits

C. Skeletal system

D. Muscular system

10


5. A teacher will use taxidermy in teaching. Which topic is suitable for taxidermy?

11

Integument is given utmost importance

Skin is preserved

Good for aquarium

Skeleton is removed in taxidermy

Muscles are removed in taxidermy

Skin covering

Feeding habits

Skeletal system

Muscular system


6. In Microtechniques, what comes last?

A. Drying, cleaning, and labeling

B. Deceration, staining and mounting

C. Washing, dehydration and clearing

D. Infiltration, embedding and sectioning

12


6. In Microtechniques, what comes last?

13

Labeling of slides

With Labeling

No Labeling

No Labeling

No Labeling

Drying, cleaning, and labeling

Deceration, staining and mounting

Washing, dehydration and clearing

Infiltration, embedding and sectioning


7. In the absence of a microtome, free hand section can be used. What cutting material is recommended?

A. Probe

B. Scalpel

C. Forceps

D. Microscope

14


7. In the absence of a microtome, free hand section can be used. What cutting material is recommended?

15

Cutting

Probe

Scalpel

Forceps

Microscope


8. Juan is a taxonomist. He correctly gives scientific names of plant specimens collected in the field. Which will greatly help Juan in naming?

A. Microtechniques

B. Herbarium

C. Aquarium

D. Terrarium

16

From Part III: Enhancing Test taking Skills-Question # 9

8. Juan is a taxonomist. He correctly gives scientific names of plant specimens collected in the field. Which will greatly help Juan in naming?

17

Classification; naming

Micro- techniques

Herbarium

Aquarium

Terrarium


9. A teacher wants to show blood samples on a slide. Which method will she use?

A. Squashing

B. Smearing

C. Teasing

D. Paraffin

18


9. A teacher wants to show blood samples on a slide. Which method will she use?

19

Liquid

Squashing

Smearing

Teasing

Paraffin


10. The following are steps in microtechinques, a procedure of making slides. Which comes first?

A. Anesthetization

B. Sectioning

C. Dissection

D. Mounting

20


10. The following are steps in microtechinques, a procedure of making slides. Which comes first?

21

Specimens must feel the least pain

Anesthe- tization

Sectioning

Dissection

Mounting


11. Which chemical is usually used in preserving specimen in microtechniques?

A. Formalin

B. Xylene

C. Water

D. Stain

22


11. Which chemical is usually used in preserving specimen in microtechniques?

23

Prevents further damage to the specimen; fixation

Preservation

Clearing

Washing; solvent

Coloration


Formalin

Xylene

Water

Stain

12. A teacher wants to show the movement of animals in his lesson. What should he use?

A. Herbarium and taxidermy

B. Terrarium and herbarium

C. Terrarium and taxidermy

D. Aquarium and terrarium

24


12. A teacher wants to show the movement of animals in his lesson. What should he use?

25

Proof of being alive

Herbarium and taxidermy

Terrarium and herbarium

Terrarium and taxidermy

Aquarium and terrarium


X

√

X

X

√

√ √

X

13. Which instrument can substitute a microtome?

A. Pin

B. Scalpel

C. Incubator

D. Thermometer

26


13. Which instrument can substitute a microtome?

27

For cuting; for sectioning


Pin

Scalpel

Incubator

Thermo- meter

14. A teacher asks students to prepare pollen grains on slides. Which skill is needed?

A. Setting up of aquarium

B. Preparing taxidermy

C. Making herbarium

D. Microtechiques

28


14. A teacher asks students to prepare pollen grains on slides. Which skill is needed?

29

Microscopic


Setting up of aquarium

Preparing taxidermy

Making herbarium

Microtechiques

15. In culturing bacteria, agar is used. Which is NOT a reason for this use?

A. liquefies at 1000C and remains liquid until about

400C

B. may be used in petri dish or test tube

C. hard to prepare in the laboratory room

D. few microbes can degrade it

30


15. In culturing bacteria, agar is used. Which is NOT a reason for this use?

31

Growing bacteria

True for agar

True for agar

False for agar

True for agar


liquefies at 1000C and remains liquid until about 400C

may be used in petri dish or test tube

hard to prepare in the laboratory room

few microbes can degrade it

16. Hay infusion is used for protist study. It has to be left for a few days before using. Why?

A. Prevents bacteria from colonizing

B. Releases energy from the protists

C. Allows growth of protist population

D. Ensures sunlight kills the unwanted organisms

32


16. Hay infusion is used for protist study. It has to be left for a few days before using. Why?

33

No protists yet in the initial setup of hay infusion

Bacteria are posssible food of the protist

Hay is the source of food for bacteria

Protist will exist once food is available for them

Sunlight will not kill the organisms


Prevents bacteria from colonizing

Releases energy from the protists

Allows growth of protist population

Ensures sunlight kills the unwanted organisms

17. Which water source can be a direct substitute for hay infusion?

A. Pond

B. Rain

C. Soil

D. Tap

34


17. Which water source can be a direct substitute for hay infusion?

35

Liquid

Liquid; usually with prostists

Liquid; no time for the protist to grow

Solid

Liquid; usually treated with chlorine


Pond

Rain

Soil

Tap

18. Which is a good technique to use in preparing blood specimens?

A. Squashing

B. Smearing

C. Grinding

D. Teasing

36


18. Which is a good technique to use in preparing blood specimens?

37

Liquid


Squashing

Smearing

Grinding

Teasing

19. Which chemical is usually used in dehydration of specimen in microtechniques?

A. Formalin

B. Alcohol

C. Xylene

D. Water

38

Additional Question

19. Which chemical is usually used in dehydration of specimen in microtechniques?

39

Removal of water

Additional Question

Formalin

Alcohol

Xylene

Water

For preservation For clearing

For washing For dehydration

20. An entire ant is chosen to be the specimen for mounting. What should be included in the label of the slide?

A. wm

B. cs

C. eg

D. ls

40

Additional Question

20. An entire ant is chosen to be the specimen for mounting. What should be included in the label of the slide?

41

Whole Additional Question

wm

cs

eg

ls

Whole mount

Cross section

Example

Longitudinal section

God bless in your LET! 42