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Institut National Polytechnique de Toulouse (INP Toulouse)

Sciences Ecologiques, Vétérinaires, Agronomiques et Bioingénieries (SEVAB)

Etude épidémiologique de la dermatose nodulaire contagieuse bovine enEthiopie et évaluation de son impact économique

mardi 29 mars 2011Getachew GARI JIMOLU

Pathologie, Toxicologie, Génétique et Nutrition

Philippe DORCHIES, Professeur, Emérite, ENVT, PrésidentJean-Pierre GANIERE, Professeur du Ministère de l'Agriculture, ENV Nantes, Membre

Stéphane BERTAGNOLI, Maître de conférences, ENVT, MembrePhilippe JACQUIET, Professeur du Ministère de l'Agriculture, ENVT, Membre

François ROGER, Chercheur, CIRAD Montpellier, Membre

Etienne THIRY, Professeur du Ministère de l'Agriculture, Université de LiègeDidier CALAVAS, Ingénieur de Recherche, AFSSA Lyon

Philippe JACQUIET, Professeur du Ministère de l'Agriculture, ENVTFrançois ROGER, Chercheur, CIRAD Montpellier

Animal et Gestion Intégrée des Risques (AGIRs), CIRAD, Montpellier

Epidemiological Study of Lumpy Skin Disease and Its Economic

Impact in Ethiopia

Getachew GARI

2011

©Copyright

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Acknowledgements

Above all, I praise the Almighty God for giving me strength to successfully complete this PhD

program.

I am highly grateful to National Animal Health Diagnostic and Investigation center for having

allowed me to join this scholarship and having kindly facilitated me with technical and material

supports during my field works. I am indebted to French Ministry of Foreign Affairs, French

Embassy in Ethiopia who granted financial support for the scholarship in the framework of PSF

No 2003-24 LABOVET project.

I would like to express my gratitude to CIRAD-EMVT for having welcomed and supported me

through all my studies. I am particularly thankful to the co-director of the thesis Dr François

ROGER for his keen help of guidance, advices and support for the successful outcome of this

program. My special thanks go to Dr Agnès WARET-SZKUTA for the coordination of all the

PhD program, technical advices and meticulous revision of my thesis documents. I am thankful

as well to Dr Fabienne BITEAU-COROLLER for her advices and help during the first year of

the study to obtain DRU and to Prof. Gérard DUVALLET for his advices and encouragements.

I would like to thank Prof. Philippe JACQUIET for his kind willingness to be the director of the

thesis and for his invaluable time dedication to review the thesis document. I am also thankful to

Prof. Jean-Pierre GANIERE, Dr Didier CALAVAS and Prof. Etienne THIRY for having kindly

accepted to be respectively the president and the reporters in the Jury.

My appreciation also goes to my friends Abdo Salem SHAIF and Ermias ALEMU for their

sincere help and support during my personal problems.

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Persistent encouragement and love of my beloved wife Aynalem GEZEHAGN has really given

me strength to pursue through all these four years. My special love goes to my children Yididia,

Dibora and Nahom GETACHEW for their patience while they felt distress in my absence.

I am grateful to everyone who has participated for the fruitful outcome of this thesis work:

Mesfin SAHLE, Philippe CAUFOUR, Sebeta NAHDIC colleagues, Zaheer NIZAMANI,

CIRAD-AGIRs staffs.

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List of publications

1. Gari, G., Tilahun, G., Dorchies, P. 2007. Study on Poultry Coccidiosis in Tiyo District, Arsi

Zone, Ethiopia. Intl. J. Poult. Sci. 7(3):251-256.

2. Gari, G., Tilahun, G., Dorchies, P. 2007. Preliminary study on Natural Resistance of Two

Chicken breeds to Acute E. tenella Experimental infection. Ethiop. Vet. J. 11(2) 25-39.

3. Gari G, Biteau-Coroller F., LeGoff C., Caufour P., Roger F., 2008: Evaluation of indirect

fluorescent antibody test (IFAT) for the diagnosis and screening of lumpy skin disease

using Bayesian method. Vet. Microbiol., 129: 269-280.

4. Gari, G., A. Waret-Szkuta, V. Grosbois, P. Jacquiet, F. Roger, 2010: Risk factors Associated

to Observed Clinical Lumpy Skin Disease in Ethiopia. Epidemiol. Infect., 138:1657-

1666.

5. Gari, G., Waret-Szkuta, A., Roger, F., Bonnet, P. Epidemiological aspects and Financial

Impact of Lumpy Skin Disease in Ethiopia. Submitted to Prev. Vet. Med.

6. Gari, G., Waret-Szkuta, A., Vladimir G., Babiuk, S., Jacquiet, P., Roger, F. Sero- prevalence

of Lumpy Skin Disease in Ethiopia (paper for publication after the defence)

Poster

Gari, G., Waret, A., and Roger, F. Qualitative Risk Assessment for Lumpy Skin Disease in

Ethiopia, Reference: ISVEE/ 220, Accepted Date: 2009-01-28.

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Résumé

La dermatose nodulaire contagieuse (DNC) est une des maladies virales les plus importantes

économiquement chez les bovins en Ethiopie. Elle est causée par le virus LSD (Lumpy skin

disease virus) appartenant au groupe des Capripoxvirus. L‟objectif de cette thèse est de mieux

comprendre l‟épidémiologie de cette maladie afin de proposer des méthodes de contrôle et de

prévention efficaces et applicables sur le terrain. Cette thèse est construite en cinq chapitres. Le

premier chapitre fait une description générale du système de production agricole en Ethiopie et

présente nos connaissances actuelles sur ce virus et cette maladie. Le second chapitre est

consacré à l‟évaluation d‟un test d‟immunofluorescence indirecte (IFI) pour le diagnostic

sérologique à l‟aide de méthodes sans gold standard. Le test de séroneutralisation virale a été

utilisé comme second test de comparaison. L‟analyse à l‟aide d‟un modèle bayesien a montré

que l‟IFI présentait une bonne sensibilité (92%) et une bonne spécificité (88%) ce qui suggère

que ce test peut être utilisé pour le diagnostic et le dépistage de masse de la DNC avec une

relativement faible proportion d‟erreurs. La possibilité de tester un grand nombre de sérums en

IFI est un autre avantage de cette technique pour conduire des études épidémiologiques de

grande envergure. La sensibilité et la spécificité de la séroneutralisation virale (SNV) étaient

respectivement de 78% et de 97%. Les deux tests IFAT et VNT ont donné des résultats

conditionnellement indépendants sur l'état de la maladie de l'animal. En conséquence, le test IFI

sera préféré pour un dépistage de masse en raison de sa meilleure sensibilité tandis que le test

SNV sera réservé à la confirmation.

Une étude épidémiologique transversale a été menée pour estimer la prévalence de la DNC

Bovine à l‟échelle du troupeau et de l‟individu et pour définir les facteurs de risque associés à

cette maladie dans le contexte particulier de l‟Ethiopie. C‟est l‟objet de la troisième partie de

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cette thèse. Un total de 330 questionnaires d‟enquêtes a été collecté de 44 associations paysannes

situées dans 15 districts. La prévalence moyenne de la DNC à l‟échelle du troupeau était de

42,8% (IC à 95% : 37,5 – 48,3). Elle était significativement plus élevée dans les zones d‟altitude

moyenne 55,2% (IC à 95% : 47,5 – 62,6) que dans les zones de basse altitude (22,3%) ou les

zones de haute altitude (43,5%). La prévalence de la DNC et la mortalité due à cette maladie,

observées à l‟échelle de l‟animal, étaient de 8,1% et de 2,12% respectivement. A nouveau, elles

étaient plus élevées dans les zones d‟altitude moyenne (10,4% et 3,2% respectivement) que dans

les zones de basse et haute altitude (P < 0,05). L‟analyse de facteurs de risque a montré que trois

variables étaient significativement associées avec la prévalence de la DNC : l‟effet de la zone

agroclimatique, la conduite de troupeaux différents sur les mêmes pâtures et les mêmes lieux

d‟abreuvement et l‟introduction de nouveaux animaux. L‟incidence maximale de la DNC était

concomitante de l‟augmentation des populations d‟insectes hématophages : cette association

dans le temps était significative (coefficient de Spearman de 0,88 ; 0,79 et 0,79 respectivement

pour les zones de haute, moyenne et basse altitude).

L‟évaluation de la faisabilité financière et des bénéfices espérés de la vaccination ont constitué la

quatrième partie de la thèse. Le coût financier à l‟échelle de la ferme des cas cliniques de DNC et

le bénéfice économique de son contrôle par la vaccination ont été analysés dans cinq districts de

la région Oromia. 747 questionnaires concernant une période de production d‟un an ont été

collectés. Des données d‟épidémiologie descriptive ont été obtenues. L‟incidence cumulée sur un

an et les taux de mortalité ont été calculés pour chaque race, sexe et groupes d‟âge. Le coût

annuel des cas cliniques de DNC a été calculé en additionnant les pertes de production dues à la

morbidité et à la mortalité. Les paramètres intervenant dans l‟estimation des coûts financiers

étaient les pertes de lait et de viande, la perte de capacité de travail (traction essentiellement) et

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les coûts de traitement et de vaccination. Le coût financier annuel par tête de bétail a été estimé à

6.43 dollars américains (USD) pour le zébu local et 58 USD pour les croisés Holstein dans les

troupeaux infectés.

Le bénéfice financier du contrôle du DNC par une année de vaccination prévue a été calculé en

utilisant l'analyse du budget partiel et les changements de la production de l'entreprise dûs à

l'intervention de contrôle et ont été mesurés à partir des variables de production de lait, de viande

et de la puissance de traction. Le taux de rendement marginal (MRR) a profité de l'intervention

de contrôle et a été estimé à 76 (7600%) et le bénéfice net par tête était de 3 USD et 33 USD

chez le zébu local et HF / bovins croisés respectivement. La réduction des coûts financiers de la

DNC par tête de bétail à l‟aide d‟un plan de vaccination annuel a été évaluée à 40% pour le zébu

local et à 58% pour les bovins croisés Holstein. L‟analyse comparative entre vaccination et

absence de vaccination a permis de montrer que les producteurs locaux pourraient non seulement

récupérer un bénéfice financier substantiel de la vaccination mais qu‟ils pourraient également

assurer la survie à long terme de leur élevage. Finalement, dans la cinquième partie sont

présentées une discussion générale de l‟étude épidémiologique et des moyens de contrôle ainsi

que les questions non résolues qui nécessitent des efforts de recherche supplémentaires. Les

résultats de l‟étude des facteurs de risque pourrait également apporter des informations utiles

pour la connaissance de l‟épidémiologie de la DNC bovine dans d‟autres pays africains.

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Summary

Lumpy skin disease (LSD) is one of economically important viral diseases of cattle in Ethiopia

caused by Lumpy skin disease virus in the member of the genus Capripox viruses. The objective

of this thesis is to better understand the epidemiological features of the disease in order to

propose practical and applicable control and prevention options. The thesis is classified in five

chapters. The first chapter describes the general agricultural production system in Ethiopia and

relates the current knowledge on the virus and the disease as given by the literature.The second

chapter deals with the performance of indirect fluorescence antibody test (IFAT) as a serological

diagnostic and screening tool that was evaluated using methods without gold standard. Virus

neutralization test (VNT) was used as the second test for comparison. The analysis of conditional

dependent Bayesian model showed that the IFAT had good accuracy both in sensitivity (92%)

and specificity (88%) parameters indicating that it could be used for LSD diagnosis and

screening (epidemiological studies, epidemiosurveillance) with less misclassification. Its

capacity to run large number of samples per plate just like ELISA could be also taken as an

advantage for large epidemiological studies. The sensitivity and specificity of VNT was 78%,

97% respectively. The two tests IFAT and VNT were found conditionally independent on the

disease status of the animal. Thus, higher sensitivity and throughput for IFAT would render the

test being selected for screening purposes and higher specificity performance of VNT would

qualify it to be used as a confirmation test.

A cross sectional study was then conducted to estimate the prevalence of LSD at herd and

animal-levels and to analyze the risk factors associated with the disease occurrence in Ethiopia.

It is presented in the third chapter. A total of 330 questionnaire surveys were collected from 44

peasant associations (PA) distributed in 15 districts. The average herd level LSD prevalence was

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42.8% (95% CI: 37.5–48.3) and it was significantly higher in the midland agro-climate 55.2%

(95% CI: 47.5–62.6) than in lowland and highland agro-climate zones (22.3% and 43.5%,

respectively). The observed LSD prevalence and mortality at animal level were 8.1% and 2.12%

respectively which were still higher in the midland zone (10.4% and 3.2%, respectively) than in

lowland and highland zones (P

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The financial benefit of controlling LSD through a one year planned vaccination was calculated

using partial budget analysis and the changes in the enterprise outputs from the control

intervention were measured from the variables milk production, beef production and draft work-

output. The marginal rate of return (MRR) gained from the control intervention was estimated at

76 (7600%) and the net benefit per head was 3 USD and 33 USD in local zebu and

HF/crossbreds cattle respectively. This implied that annual vaccination had enabled to reduce the

financial costs due to LSD by 40% and 58% per head in local zebu and HF/crossbreds

respectively. The analysis of the planned vaccination as compared to a non vaccination scenario

for a one year time horizon have shown that the livestock producers would get substantial benefit

not only from financial gain perspective but also to secure and maintain sustainable farm

business. Finally in the fifth chapter, general discussion on the epidemiological study and control

options were presented along with persistent knowledge gaps that requires further research

efforts to fine-tune the proposed control and prevention options. The result from the risk factor

analysis could also shed light on the epidemiology of LSD in other African countries suffering

from the disease.

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Table of Contents

Acknowledgements ....................................................................................................................2

List of publications ....................................................................................................................4

Résumé ......................................................................................................................................5

Summary ...................................................................................................................................8

Chapter I. Literature Review ........................................................................................................ 17

1. General Introduction .................................................................................................... 18

2. Pox viruses of Vertebrates ............................................................................................ 22

3. Diseases caused by Capri-poxviruses (CaPV) ............................................................. 26

4. Lumpy Skin Disease (LSD) .......................................................................................... 29

History of LSD .................................................................................................................. 29

Etiology............................................................................................................................. 30

Geographical distribution .................................................................................................. 31

Epidemiology and pattern of the disease ............................................................................ 33

Mode of Transmission and Host Range ............................................................................. 35

Clinical Signs and Pathogenesis......................................................................................... 37

Pathological lesions ........................................................................................................... 39

Diagnosis .......................................................................................................................... 40

5. Control and Prevention ................................................................................................ 44

Vaccines for LSD control .................................................................................................. 44

New Recombinant Vaccines .............................................................................................. 46

Control and Eradication in Disease free countries .............................................................. 47

6. Economic Importance .................................................................................................. 47

7. The Objective and goals of the PhD research .............................................................. 48

General objective............................................................................................................... 48

Specific objectives ............................................................................................................. 49

Chapter II. Article 1 : Evaluation of indirect fluorescent antibody test (IFAT) for the diagnosis and screening of lumpy skin disease using Bayesian method .................................. 51

Chapter III. Article 2: Risk factors associated with observed clinical lumpy skin disease in Ethiopia .......................................................................................................................................... 68

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Chapter IV. Article 3: Epidemiological aspects and Financial Impact of Lumpy Skin Disease in Ethiopia............................................................................................................................ 87

Introduction ......................................................................................................................... 94

Materials and Methods ....................................................................................................... 96

Study site and sampling method ........................................................................................ 96

Field data collection .......................................................................................................... 97

Data analysis ..................................................................................................................... 98

Financial impact of the LSD outbreak at farm level ........................................................... 99

Partial budget analysis: financial benefit of LSD control .................................................. 102

Results ................................................................................................................................ 103

Description of cattle production system ........................................................................... 103

Financial impact of an LSD outbreak at farm level .......................................................... 105

Financial benefit of LSD control by vaccination .............................................................. 106

Discussion .......................................................................................................................... 106

Acknowledgement ............................................................................................................. 110

References .......................................................................................................................... 111

Chapter V. General Discussion and Perspectives..................................................................... 124

Epidemiology of Lumpy skin disease in Ethiopia ............................................................ 125

Risk factors associated to LSD occurrences ..................................................................... 128

Financial impact of LSD in infected herds and the benefit of its control ........................ 131

Recommendations ............................................................................................................. 134

References.............................................................................................................................. 137

Appendices ............................................................................................................................ 149

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List of Figures

Figure 1: Long term average annual rainfall (mm); Source: Alemayehu, 2009 ........................... 20

Figure 2: Phylogeny tree (NJ tree) of poxviruses based on concatenated amino acid sequences

from 29 conserved orthologous proteins (13, 475 aligned sites); Source: Hughes et al., 2010 .... 25

Figure 3: Distribution of Sheeppox and Goatpox diseases in the World. The arrows show the

recent outbreak reported parts of the world; Source: Babiuk et al., 2008a .................................. 27

Figure 4: Geographical distribution of LSD; Source: Lefèvre, P C, Gourreau, J M, 2010........... 29

Figure 5: Number of LSD outbreaks reported based on outbreak notification reports from year

2000- 2009 ................................................................................................................................ 33

Figure 6: Clinical case of LSD in Dawa-Chefa District in 2008 (Ethiopia). Circumscribed

nodules on the skin all over the body and swollen superficial lymphnodes (A,B)....................... 38

Figure 7: Vasculitic necrosis with cell debris and severe diffuse infiltration with inflammatory

cells mainly neutrophils, are seen in the superficial and deep dermis; Source: Brenner et al.,

2006. ......................................................................................................................................... 40

Figure 8: A Capripox virion from the skin of Capripox infected goat; the virus particle is

indicated by arrow; Source: Babiuk et al., 2008a ....................................................................... 41

Figure 9: Simple illustration of Bayesian model to generate the inference mean or median values

................................................................................................................................................. 54

Figure 10: The sensitivity, specificity and prevalence estimations by Bayesian model ............... 55

Figure 11: Questionnaire survey results of seasonal increase in biting-fly activity vs. lumpy skin

disease (LSD) occurrence. ......................................................................................................... 73

Figure 12: Biting fly population density through the year in 2008/2009 based on fly catchment.

................................................................................................................................................. 73

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Figure 13: LSD study locations and major agro-ecological zones in Ethiopia .......................... 128

Figure 14: Interactions between the risk factors for LSD transmission and spread that possibly

leading to the disease occurrence ............................................................................................. 131

List of Tables

Table 1: Classification of Poxviruses of vertebrates: Subfamily Chordopoxvirinae ................... 23

Table 2: Poxviruses of veterinary importance that affect domestic and laboratory animals;

Source: Fenner et al., 1987 ........................................................................................................ 24

Table 3: Summary of monthly Biting Fly density per trap per day Records (Flies/trap/day) ....... 86

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List of Abbreviations

AGID Agar gel immunodiffusion

CaPV Capripox virus

CI Confidence interval

CIRAD Centre International de Recherche Agronomique pour le Développement

CPE Cytopathic effect

CSA Central Statistics Authority

CuI Cumulative incidence

DNA Deoxyribonucleic acid

ELISA Enzyme linked immune sorbent assay

FAO World Food Organization

FITC Fluorescein Isothiocyanate

FMD Foot and mouth disease

GDP Gross domestic product

GP Goat pox disease

GTPV Goat pox virus

HF Holstein Friesian

IFAT Indirect fluorescent antibody test

IgG Immunoglobulin G

KS-1 Kenyan sheep pox strain 1

LSD Lumpy skin disease

LSDV Lumpy skin disease virus

m.a.s.l. meter above sea level

MLE Maximum likelihood estimate

mm millimeter

MoARD Ministry of Agriculture and Rural Development

mRNA messenger ribonucleic acid

MRR Marginal return rate

NB Net benefit

nm nanometer

NVI National Veterinary Institute

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OA3.Ts lamb testis cell line

OIE Office International des Epizooties, World Animal Health

OR Odds ratio

PA Peasant Association

PARC Pan African Rinderpest Campaign

PBS Phosphate-buffered saline

PCR Polymerase Chain Reaction

PPR Peste des Petits Ruminants

RVF Rift valley fever

Se Sensitivity

SGPV Sheep goat pox virus

SNNPR Southern nation nationalities and peoples region

SP Sheep pox disease

Sp Specificity

SPPV Sheep pox virus

SSDP Small scale dairy production

TCID50 Tissue culture Infective dose 50%

TCV Total costs that vary

TLU Tropical Livestock unit

USD United States of Americas‟ Dollar

UV Ultra violet

Vero-cells African green monkey kidney cells

VNT Virus neutralization test

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Chapter I.

Literature Review

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Literature Review 1. General Introduction

Ethiopia’s topography

Ethiopia is located in Eastern Africa. It borders Sudan on the West, Eritrea on the North,

Djibouti and Somalia on the East and Kenya on the South. The total area of the country is

1,127,127 square kilometers. The capital city is Addis Ababa, which is located in the center of

the country.

Ethiopia‟s topography consists of a central high plateau bisected by the Ethiopian segment of the

Great Rift Valley into northern and southern highlands and surrounded by lowlands, more

extensive on the east and southeast than on the south and west. The plateau varies from 1500 –

3000 meters above sea level (m.a.s.l.). The highest mountain point is Ras Dashen at 4620 m.a.s.l.

in the northern highlands. In the eastern part of the rift valley, the Denakil depression is 115

meters below the sea level and is one of the hottest places on earth. The diversity of Ethiopia‟s

terrain determines regional variations in climate, natural vegetation, soil composition and

settlement patterns (Anon., 2005; Alemayehu, 2009).

Climate

Altitude-induced climate conditions form the basis for three climatic zones: cool, temperate and

hot which have been known to Ethiopians as Dega, Weinadega and Kola respectively. The cool

zone (highland) above 2300 m.a.s.l. has a temperature ranging from 16°C to near freezing. A

temperate zone with a daytime temperature between 16°C- 30°C occurs in the mid highland zone

ranging from 1500 m.a.s.l. to 2300 m.a.s.l. In areas below 1500 m.a.s.l. classified as lowlands,

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such as the rift valley, the southeast, the southern and western border lands, daytime temperature

ranges from 30°C to over 50°C in Denakil depression (Anon., 2005; Alemayehu, 2009).

Precipitations are determined by differences in elevation and by seasonal shifts in monsoon

winds. The highlands receive by far the most rainfall than lower elevations. Rainfall has two

major seasons: the Belg, a lighter rainy season that usually begins in mid-February and continues

up to end of April and the Kiremt, the major rainy season starting mid-June and ending mid-

September. In general, relative humidity and rainfall decrease from south to north and are always

meager in the eastern and south-eastern lowlands (Figure 1) (Alemayehu, 2009).

Population

The Ethiopian administrative structure encompasses 9 regions and 2 city administrations that

include about 546 districts. Each district is composed of a different number of Kebeles which are

the lower administrative level in Ethiopia. In 2008, estimated Ethiopia‟s population was about 80

million (CSA, 2006). The annual population growth rate is estimated at 2.6%. The population is

concentrated in the northern and southern highlands. The lowlands in the southeast, south and

west are mostly being sparsely populated.

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Figure 1: Long term average annual rainfall (mm); Source: Alemayehu, 2009

The agricultural sector

Agriculture is the cornerstone of Ethiopia‟s economy on which 84% of the rural populations

sustain their livelihood. The agricultural production system is mainly a sedentary mixed crop-

livestock production system in the midlands and highlands whereas in most of lowlands semi-

pastoral and pastoral production systems are dominant (herd owners move their animals

seasonally in search of feed and water sometimes over a long distance) (Alemayehu, 2009). The

crops grown vary according to the soil types and altitude variations. The main cereal staples are

wheat, barley, teff (Eragrostis abyssinica), maize and sorghum. Cash crops include coffee,

oilseeds and spices.

Livestock production is an integral part of the country‟s agricultural system. The livestock

subsector accounts for 40% of the agricultural gross domestic product (GDP) and 20% of the

total GDP without considering other contribution like traction power, fertilizing and mean of

transport (Aklilu et al., 2002). In 2004 the livestock sector has contributed around 12% of the

total foreign currency earning (Anon., 2009). Livestock are significant components of small

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scale mixed crop livestock production systems. Draft-oxen are used for ploughing to produce

crops. Manure is the cheapest and easily available fertilizer to increase soil fertility. In the

lowland parts of Ethiopia, the livelihood of pastoralists and semi-pastoralists relies on livestock

production for their food, income source, cultural and social prestige. Common grasslands

provide extensive pasture and browse in most parts of the country. Animals are free-ranging in

the communal grazing fields and different species are herded together. Natural grass, post-

harvest crop residuals and straw are the main source of feed. Concentrate feeds and feed-

additives are seldom used (Alemayehu, 2009).

The livestock population is estimated at 48.9 million tropical livestock units (TLU) which

includes 41.5 million cattle, 14.6 million sheep, 13.7 million goats, 5.8 million equids, 447 842

camels and 43 million chickens (CSA, 2006). The main cattle breeds classified based on

genetical and geographical locations are the Arsi (highland zebu), Boran, Fogera, Horo, Sheko

(Gimira), Nuer (Abigar) and Adal (Afar). The Fogera and Horo are well known for their milk

production and reared around Lake Tana and in the East Wellega Zone respectively. The Boran,

a dual purpose breed, is found in the southern and eastern part of the country. The Sheko and

Nuer breeds in the Southwest and Sheko breed is considered to have tolerance to high tse-tse

challenge (Lemecha et al., 2006). Exotic breeds such as Holstein Friesian and Jersey have been

imported and used for cross breeding with the indigenous cattle (Alemayehu, 2009).

Animal Health

The major cause of economic losses and of poor productivity in livestock is the prevalence of a

wide range of diseases such as Contagious Bovine Pleuropneumoniae (CBPP), Foot and Mouth

Disease (FMD), Lumpy Skin Disease (LSD), Contagious Caprine Pleuropneumoniae (CCPP),

Peste des Petits Ruminants (PPR), African Horse Sickness (AHS), Trypanosomosis and the

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presence of internal and external parasites. In general animal diseases are considered to account

for 50 to 60% decrease in productivity per year by retarded growth, low fertility, decreased milk

production and work output, increased mortality, and by restricting the introduction of more

productive exotic breeds. The losses due to mortality are estimated to range from 4-7% for cattle,

7-11% for sheep and 7-11% for goats per annum (Abraham Gopilo, 2005). Other major impacts

of livestock diseases are the consequences from sanitary barrier to livestock export trade and

direct human losses in case of zoonosis (disease transmissible from animal to human). Public

sector expenditures on the control of these livestock diseases, for surveillance and monitoring

would also constitute a substantial economic loss for the country as the money used could have

been allocated for other developmental purposes (Rich and Perry, 2010).

2. Pox viruses of Vertebrates

Eight genera are found within the Chordopoxvirinae subfamily of the Poxviridae (Table 1 and

2). The members of this family are among the largest of all viruses, brick shaped or ovoid virions

measuring 220-450 nanometer (nm) by 140-266nm. The virions have an external coat containing

lipid and an irregular arrangement of tubules on the outer membrane in most genera except the

Parapox viruses that have regular spiral arrangement of “tubules” on the outer membrane

(Fenner et al., 1987; Sharma and Adlakha, 1995). The virions contain about 30 structural

proteins and several enzymes. The nucleic acid is a double stranded Deoxyribo Nucleic Acid

(DNA) of molecular weight in the range between 150 and 240*106 daltons. The evolutionary

biology of the poxviruses, phylogeny, with particular emphasis on transfer of poxviruses across

host species boundaries were reviewed (Xing et al., 2006; Hughes et al., 2010) (Figure 2). The

multiplication takes place in the cytoplasm and the cytoplasmic accumulations produce A type

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inclusion bodies (Fenner et al., 1987; Coetzer et al., 1994; Sharma and Adlakha, 1995;

Bertagnoli and Séverac, 2010).

The members of some genera are ether resistant while other genera are ether sensitive. The pox

viruses withstand drying for months and even storage at room temperature. They are destroyed

by moist heat at 60o C within 10 minutes. They are also resistant to many common disinfectants

(Fenner, et al., 1987). The spread of infection occurs by the respiratory route or through the skin.

Some members are also mechanically transmitted by arthropods (Fenner et al., 1987; Coetzer et

al., 1994; Sharma and Adlakha, 1995; Bertagnoli and Séverac, 2010).

No Genera Prototype virus

1 Orthopox virus Vaccinia

2 Parapox virus Orf virus

3 Capripox virus Sheep pox virus

4 Suipox virus Swine pox virus

5 Leporipox virus Myxoma virus

6 Avipox virus Fowl pox virus

7 Yatapoxvirus Yaba monkey tumor virus

8 Molluscipoxvirus Molluscum contagiosum virus

Table 1: Classification of Poxviruses of vertebrates: Subfamily Chordopoxvirinae

http://en.wikipedia.org/wiki/Yatapoxvirushttp://en.wikipedia.org/wiki/Yaba_monkey_tumor_virushttp://en.wikipedia.org/w/index.php?title=Molluscipoxvirus&action=edit&redlink=1http://en.wikipedia.org/wiki/Molluscum_contagiosumhttp://en.wikipedia.org/w/index.php?title=Chordopoxvirinae&action=edit&redlink=1

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Genus Virus Animals naturally

affected

Host

range

Geographical

Distribution

Parapoxvirus Pseudocowpox virus Cattle, human Narrow Worldwide

Bov. Papular stomatitis

virus

Cattle, human Narrow Worldwide

Orf virus Sheep, goat, human Narrow Worldwide

Capripoxvirus Sheeppox virus Sheep, goat Narrow Africa, Asia

Goatpox virus Goat, Sheep Narrow Africa, Asia

LSD virus Cattle, buffalo Narrow Africa

Suipoxvirus Swine pox virus Swine Narrow Worldwide

Leporipoxvirus Myxoma virus, Hare

fibroma virus, Rabbit

fibroma virus, Squirrel

fibroma virus

Rabbit

Hare

Squirrel

Narrow Americas,

Europe,

Australia

Avipoxvirus Fowlcholera virus,

Canary pox virus, Pigeon

pox virus, Turkey pox

virus,Quailpox virus

Chickens, turkey,

other birds

Narrow Worldwide

Orthopoxviruses Vaccinia virus Human, cow,

buffalo, pig, rabbit

Broad Worldwide

Cowpox virus, Buffalo

pox virus

Cow, human,

numerous spp.

Broad Europe

Asia

Ectromelia virus, Rabbit

pox virus

Mice

Rabbit

Narrow Europe

Monkeypox virus Monkeys, Squirrel,

many others

Broad West and

Central Africa

Uasin Gishu virus Horse Broad East Africa

Table 2: Poxviruses of veterinary importance that affect domestic and laboratory animals;

Source: Fenner et al., 1987

http://en.wikipedia.org/w/index.php?title=Hare_fibroma_virus&action=edit&redlink=1http://en.wikipedia.org/w/index.php?title=Hare_fibroma_virus&action=edit&redlink=1http://en.wikipedia.org/w/index.php?title=Rabbit_fibroma_virus&action=edit&redlink=1http://en.wikipedia.org/w/index.php?title=Rabbit_fibroma_virus&action=edit&redlink=1http://en.wikipedia.org/w/index.php?title=Squirrel_fibroma_virus&action=edit&redlink=1http://en.wikipedia.org/w/index.php?title=Squirrel_fibroma_virus&action=edit&redlink=1

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Figure 2: Phylogeny tree (NJ tree) of poxviruses based on concatenated amino acid sequences

from 29 conserved orthologous proteins (13, 475 aligned sites); Source: Hughes et al., 2010

The tree was constructed on the basis of the JTT amino acid distance, assuming that rate

variation among sites follows a gamma distribution (shape parameter a = 0.86). Numbers on the

branches represent percentages of 1000 bootstrap samples supporting each branch; only values ≥

50 % are shown (Hughes et al., 2010).

http://www.ncbi.nlm.nih.gov/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=An external file that holds a picture, illustration, etc.Object name is nihms152125f1.jpg [Object name is nihms152125f1.jpg]&p=PMC3&id=2818276_nihms152125f1.jpg

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Viral replication

Replication of poxvirus occurs in the cytoplasm. After fusion of the virion with the plasma

membrane or via endocytosis, the viral core is released into the cytoplasm. Transcription is

initiated by viral transcriptase and functional capped and polyadenylated messenger Ribonucleic

Acid (mRNAs) are produced within minutes after infection. The polypeptides produced by

translation of these mRNAs complete the uncoating of the core and about half of the viral

genome is transcribed prior to replication, comprising genes encoding proteins involved in host

interactions, viral DNA synthesis, and intermediate gene expression. With the onset of DNA

replication 1.5 to 6 hours after infection, there is a dramatic shift in the gene expression and

almost the entire genome is transcribed, but transcripts from the early genes (i.e. those

transcribed before DNA replication begins) are not translated. Two forms of virions are released

from the infected cells (virions with one membrane, and virions with two membranes) and both

types are infectious (Fenner et al., 1987; Bertagnoli and Séverac, 2010).

3. Diseases caused by Capri-poxviruses (CaPV)

Capripoxviruses (CaPVs) represent one of the eight genera within the Chordopoxvirinae

subfamily of the Poxviridae. The capripoxvirus genus is comprised of Lumpy skin disease virus

(LSDV), Sheeppox virus (SPPV), and Goatpox virus (GTPV). These viruses are responsible for

some of the most economically significant diseases of domestic ruminants in Africa and Asia.

CaPV infections have specific geographic distributions (Davies, 1991; Coetzer et al., 1994).

Sheeppox and Goatpox viruses are endemic throughout southwest and central Asia, the Indian

subcontinent, and northern and central Africa (Figure 3). In contrast, LSDV occurs largely in

southern, central, eastern and western Africa with a few sporadic reports in the Middle East

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Asian countries (Figure 4) (Bhanuprakash et al., 2006; Babiuk et al., 2008a; ANON., 2010;

Fassi-Fehri, 2010; Lefèvre and Gourreau, 2010).

Figure 3: Distribution of Sheeppox and Goatpox diseases in the World. The arrows show the

recent outbreak reported parts of the world; Source: Babiuk et al., 2008a

CaPVs are, however, serologically indistinguishable from each other. Restriction enzyme

analysis or partial and complete DNA sequence data also support a close relationship between

CaPVs (Gershon and Black, 1987; Kitching et al., 1989). CaPVs are generally considered to be

host specific (Capstick and Coackley, 1961a). This has been shown specifically for Nigerian,

Middle Eastern, and Indian strains of SPPV and GTPV and for LSDV (Stevenson et al., 2000).

However, the ability of SPPV and GTPV strains to naturally or experimentally cross-infect and

cause disease in both host species has been described previously (Davies, 1982). They are able to

induce heterologous cross-protection (Carn, 1993; Barnard et al., 1994).This similarity between

Sheeppox and Goatpox has led to the suggestion that they are part of a disease complex caused

by a single viral species and that observable host range specificities result of regional virus

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adaptations to sheep or goat hosts. However, restriction endonuclease analysis and cross-

hybridization studies of SPPV and GTPV indicate that these viruses, although closely related

(estimated 96 to 97% nucleotide identity), can be distinguished from one another and may

undergo recombination in nature (Tulman et al., 2002). SPPV and GTPV DNA sequence

analysis also indicate a high degree of similarity to LSDV, which genome sequence contains a

conserved ChPV-like complement of replicative genes and a unique complement of virulence

and host range genes (Gershon and Black, 1987; Kitching et al., 1989; Tulman et al., 2002).

Moreover, restriction enzyme analyses of the genome of the Kenya SGPV and LSDV strains

have shown that they appear to be identical (Kitching et al., 1987; Davies, 1991; Tulman et al.,

2001; Le Goff et al., 2009).

CaPVs induce highly economic important diseases of sheep, goat and cattle causing significant

production losses in endemic countries. Sheep pox and goatpox cause reduced milk production,

decreased weight gain, abortion, damage to wool and skin, increased susceptibility to pneumonia

and fly strike and mortality (Bhanuprakash et al., 2006). A production loss by LSD is also

similar in cattle causing skin damage with occasional fatality. Should CaPVs diseases be

introduced into the countries where the diseases are exotic, the economic costs because of trade

restrictions and the need of disease eradication would be substantial and comparable to a Foot

and Mouth disease outbreak (Babiuk et al., 2008a). Capripox diseases are considered as

transboundary diseases which have significant impendent on livestock market and animal

products. In addition Capripoxviruses are listed by the US Department of Agriculture as Select

Agents Legislation on the National Select Agent Registry List and are considered as potential

economic bioterrorism agents (Babiuk et al., 2008a).

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Figure 4: Geographical distribution of LSD; Source: Lefèvre, P C, Gourreau, J M, 2010

4. Lumpy Skin Disease (LSD)

LSD is an acute to sub acute viral disease of cattle that can cause mild to severe

symptoms including fever, nodules in the skin, in the mucous membranes and in the internal

organs, skin oedema, lymphadenitis and sometimes death. The disease can result in economic

losses due to decreased milk production, traction power loss, weight loss, poor growth, abortion,

infertility and skin damage. Pneumonia is a common sequel in animals with lesions in the mouth

and respiratory tract (Davies, 1991; OIE, 2010).

History of LSD

The clinical syndrome of LSD was first described in Zambia (formerly Northern Rhodesia) in

1929. Between 1943 and 1945, cases occurred in Botswana (Bechuanaland), Zimbabwe

(Southern Rhodesia) and the Republic of South Africa. The infectious nature of the disease was

recognized at this time. A panzootic in South Africa, which lasted until 1949, affected some

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eight million cattle and consequently incurred enormous economic losses (Diesel, 1949; Davies,

1991).

LSD was first identified in East Africa in Kenya in 1957 and Sudan in 1972, then in West Africa

in Nigeria in 1974, and it was reported in 1977 in Mauritania, Mali, Ghana, and Liberia (OIE,

2010). Another epizootic of LSD between 1981 and 1986 affected Tanzania, Kenya, Zimbabwe,

Cameroon, Somalia and Ethiopia. In May 1988, LSD was recognized clinically in the Suez

Governorate of Egypt, where it was thought to have arrived at the local quarantine station with

cattle imported from Africa. The disease spread locally in the summer of 1988 and apparently

overwintered with little or no manifestation of clinical disease. It reappeared in the summer of

1989 and, in a period of five to six months, spread to 22 of the 26 governorates of Egypt (Ali et

al., 1990).

In 1989, a focus of LSD was identified in Israel and subsequently eliminated by the slaughter of

all infected cattle as well as contacts. But another outbreak reappeared recently in 2006

(Yeruham et al., 1995; Brenner et al., 2006). LSD has continued to be reported from the Middle

East countries, Palestinian Autonomous Territory and Oman since 2006 (OIE, 2010). Cases have

also been reported in Yemen (OIE, 1990). Sporadic reports were recorded in Kuwait in 1986-

1988 (OIE, disease report, 1988), Bahrain in 1993 (not confirmed by virus isolation), La Réunion

Island in 1993, Mauritius in 2000 (Barnard et al., 1994; Lefèvre and Gourreau, 2010).

Etiology

LSD is caused by Lumpy Skin Disease virus (LSDV) within the genus Capripoxvirus and the

prototype strain is Neethling Virus. It is an enveloped DNA virus, ovoid shape with a molecular

size of 350*300nm and a molecular weight that ranges from 73 to 91 (Kilodalton) KDa. LSDV

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genome sequences were assembled into a contiguous sequence of 150.8 kilobase pair (kbp)

which is in accordance with previous size estimates of 145 to 152 kbp (Tulman et al., 2002; Kara

et al., 2003). These genes encode several poxviral proteins known to be structural or involved in

virion morphogenesis and assembly. The terminal genomic sequences contain a unique

complement of at least 34 genes which are responsible in virulence, host range and/or immune

evasion (Tulman et al., 2002; Johnston and McFadden, 2003; Kara et al., 2003). LSDV is

genetically and antigenically closely related to a strain of sheep and goat pox virus (Alexander et

al., 1957). Comparison of LSDV genome with published restriction fragment analysis of the

SPPV and GTPV genome indicates that there may be additional terminal sequences of less than

200 bp present (Gershon and Black, 1987; Kitching et al., 1989; Tulman et al., 2002).

LSDV is susceptible to sun light and detergents containing lipid solvents. The virus could be

inactivated after heating for 1 hour at 55°C (Davies and Otema, 1981; Coetzer et al., 1994;

Lefèvre and Gourreau, 2010). However, it withstands drying, pH changes if not an extreme pH

and can remain viable for months in dark room such as infected animal shade off its host. LSDV

can persist in skin plugs for about 42 days (Babiuk et al., 2008b; Lefèvre and Gourreau, 2010). It

is likely that the viral A type inclusion body protein in infected cells may protect the virion after

the scab has disintegrated, although this has not yet been proven (Babiuk et al., 2008a).

Geographical distribution

LSD distribution has extended from sub-Saharan countries to Egypt and Western Africa.

Outside the African continent Israel has reported LSD outbreaks and sporadically some Middle

East countries which showed that there is a real potential risk of the disease to establish

endemically there (Brenner et al., 2006). Epidemiological trend of LSD suggests that there could

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also be a considerable potential risk of the disease spreading further into North Africa, into the

Middle East countries and to Mediterranean regions because of global climatic changes and trade

movement in animals and animal products (Davies, 1991; Babiuk et al., 2008a).

In Ethiopia, LSD was first observed in 1983 in the western part of the country (southwest

of Lake Tana) (Mebratu et al., 1984). After its first appearance, an explosive sudden epidemic

spread from the north through the central to the southern part of the country. In the subsequent

three to five years, it had covered the vast area of the highland and midland parts of the country.

LSD is one of reported diseases in Ethiopia which deserves outbreak notification to the National

veterinary services. However, a variable degree of under-reporting of the outbreak cases could

exist from different parts of the country. Data investigations from the national disease outbreak

report database during the period 2000-2009 showed that major epidemic outbreaks of LSD

occurred in 2000/2001 in the northern parts of the country in Amhara and West Oromia regions.

Then it extended to the central and the southern parts of the country in 2003/04 covering large

parts of Oromia and Southern Nation, Nationalities and Peoples (SNNP) regions. In 2006/07

another extensive outbreak reappeared in Tigray, Amhara and Benishangul regions in the

northern and north-western parts of the country. From 2007 up to 2009 the outbreak number

progressively increased in Oromia Region situated in the central part of the country while it

seemed to be gradually decreasing in the northern part of the country including Tigray, Amhara

and Benishangul regions. This showed that an epidemic reoccurs after an interval of 5-6 years

cycle in unvaccinated cattle population. The national disease outbreak report during these 10

years showed that LSD has spread virtually to all the regions in the country and in different agro-

climatic zones (Figure 5) (MoARD, Epidemiology Section Personal communication).

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Studies based on clinical disease observation done around Nekemt town, Wolliso town and in

Southern rangeland in Ethiopia have reported different animal level prevalence of LSD ranging

from 7 to 28% (Asegid, 1991; Beshahwured, 1991; Regassa, 2003). A mortality of 1-3% was

observed in the same study and was similar to a previous report by Davies (1991). However,

epidemiological studies carried out in Ethiopia to date were of limited scopes and did not

elucidate the full image of its distribution in the country.

Figure 5: Number of LSD outbreaks reported based on outbreak notification reports from

year 2000 to 2009

Epidemiology and pattern of the disease

The incubation period of LSD is 6 to 10 days in experimentally infected animals (Babiuk et al.,

2008b) but is thought to be 2 to 4 weeks in naturally-infected animals (Barnard et al., 1994). The

World Organization for animal health (OIE) Code gives the maximum incubation period of 28

days for regulatory purposes.

188172 168

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100

150

200

250

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utb

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s R

epo

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The morbidity of LSD varies enormously and is usually estimated at 10% in endemic areas

which may be contrasted with those of 80 to 90% in different situations like in South Africa

(Barnard et al., 1994; Babiuk et al., 2008a). In southern, West and East Africa, higher

morbidities have been encountered in epizootics, yet much lower morbidity may also occur

during other epizootics (Davies, 1991). Mortality of 10 to 40% and even higher have been

reported on occasion but the lower range of 1 to 5% is more usual (Davies, 1991; Barnard et al.,

1994; Babiuk et al., 2008a). The reason why morbidity and mortality enormously vary during the

epizootic of LSD infection is not yet clearly known, but different factors attributed to this

variation are cattle breed, health status of the animal, viral isolates and insect vectors involved in

the transmission. Thus in general, the breeds of Bos taurus, imported into Africa from Europe, or

Australia are far more susceptible than the indigenous Bos indicus cattle (Davies, 1991; Barnard

et al., 1994; Babiuk et al., 2008a). Diseases and factors which compromise the immune status of

the animal such as trypanosomosis in the western part of Ethiopia might add to the severity of

LSD infection. The distribution and relative abundance of insect vectors are also thought to

reflect the differences in morbidity rates in the various habitats. Finally, mechanical insect

vectors which are capable to pierce deep in to the tissue feeding from intravenous blood are

assumed to cause severe clinical LSD (Kitching and Mellor, 1986; Carn and Kitching, 1995;

Chihota et al., 2001).

LSD occurs in many different biotopes from the temperate high altitude through to the various

wet and dry savannah ecotypes and the dry semi-arid and thorn scrub. It can also spread

extensively in irrigated lands like in Sudan and Egypt (Davies, 1991). The disease usually occurs

during wet seasons and shortly after the major rainy season. It has a feature that an epidemic

reoccurs after an interval of 5-6 years in susceptible cattle population (Woods, 1988; Barnard et

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al., 1994). However, further study of the risk factors associated with the disease occurrence is

needed.

Mode of Transmission and Host Range

The virus of LSD does not spread readily among animals held in insect-proof pens. While

infection by contact can occur, it is not considered a major component of transmission during

epizootics (Carn and Kitching, 1995). Most infection is thought to be the result of blood sucking

arthropods mechanically (Thomas and Mare, 1945; Von Backstrom, 1945; Diesel, 1949;

MacOwan, 1959; Kitching and Mellor, 1986; Chihota et al., 2001). The multiplication of LSDV

in the vector insects has not been demonstrated. In the infected animal virus is present in blood,

nasal and lachrymal secretions, semen and saliva, which may be sources for transmission (Irons

et al., 2005; Babiuk et al., 2008b). LSD is transmissible to suckling calves through infected milk.

Direct transmission can occur when the animals share the same drinking trough due to

contamination by nasal and salivary discharges from infected animals (Barnard et al., 1994;

Lefèvre and Gourreau, 2010). The virus enters the host either through the skin or the digestive

tract mucosa.

Particular types of insects incriminated in the transmission of LSDV are not all elucidated. Virus

has been isolated from Stomoxys species and Biomyia fasciata species commonly associated with

cattle and found in large numbers during LSD epizootics (Weiss, 1968). S. calcitrans has been

thought as the most likely insect to have a role in the epidemiology of LSD based on the

detection and isolation of virus from flies that had fed on infected cattle during an outbreak

(Diesel, 1949; MacOwan, 1959; Anon., 2008). Stomoxys spp have been shown to transmit

SGPV successfully (Kitching and Mellor, 1986). In 1989 the LSD outbreak in Israel was

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attributed to infected S. calcitrans carried over by wind from Ismailiya in Egypt (Yeruham et al.,

1995). The introduction of LSD to La Réunion in 1991 was also exclusively attributed to

Stomoxys despite all the official quarantine and prohibition of cattle movement measures were

implemented (Lefèvre and Gourreau, 2010). However, there are still doubtful issues on this

assumption which could raise some questions on the very nature of mechanical transmission that

requires short time period to transmit the pathogens, and the distance that these flies could be

blown by wind, if any because of the large size of Stomoxys flies which might unlikely be able to

blow by wind like mosquitoes to far distances. In an experimental transmission attempt, Aedes

aegyti (Diptera: Culicidae) was reported to transmit LSDV in cattle (Chihota et al., 2001)

whereas the transmission by Stomoxys spp. was not successful (Chihota et al., 2003). Other

biting flies like Tabanids, Glossina spp, Culicoides spp have been suspected to be involved. The

potential of Ixodid ticks to transmit LSDV was also reported (Tuppurainen et al., 2010). An

embarrassing gap in our knowledge requires defining the transmission mechanisms of LSD and

research efforts are required to understand the prevalence of the different biting flies potentially

associated with LSDV transmission in the various biotypes of countries.

Some wild species like Giraffe (Giraffa camelopardalis), Impala (Aepyceros melampus), and

Thomson's gazelle have been infected experimentally by parenteral inoculation with LSDV and

have developed characteristic lesions. However, under natural conditions, lesions of LSD have

not been seen on these animals when they have been present during epizootics of the disease

(Young et al., 1970). Sheep and goats do not become infected during outbreaks of LSD even

when held in close contact with infected cattle. African buffaloes (Syncerus caffer) and Asian

water buffaloes (Bubalus bubalis) do not show lesions in the field during epizootics of LSD but

both buffalo types may suffer an unapparent infection and seroconvert (Davies, 1991). In an

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enzootic area of LSD in Kenya, many African buffaloes had high titers of antibodies to Capripox

virus whereas in another area, no antibody was found (Davies, 1991). Infection has been reported

in Arabian Oryx in Saudi Arabia (Greth et al., 1992). In general the role of wildlife in the

transmission and maintenance of LSDV was found almost negligible (Hedger and Hamblin,

1983). The absence of reservoir host for LSD virus might lead us to the assumption that infection

might persist in the endemic areas at a low level as unapparent or mild form in the cattle

population (Woods, 1988; Lefèvre and Gourreau, 2010).

Clinical Signs and Pathogenesis

The characteristic clinical signs of LSD are a fever of 40–41.5oC that may last 6–72 hours

and occasionally up to 10 days which is accompanied by watering eyes, increased nasal and

pharyngeal secretions, loss of appetite, reduction in milk production, some depression and

reluctance to move.

Within 1–2 days onset of the clinical signs there is a cutaneous eruption of nodules or lumps,

which may cover the whole of the body. The most common sites are the head and neck,

perineum, genitalia and udder, and the limbs. The nodules are 0.5–5 cm in diameter, appearing as

round circumscribed areas of erect hair, firm and slightly raised from the surrounding skin

(Figure 6). The lesions are full skin thickness involving the epidermis, dermis and subcutis,

which may be oedematous. Regional lymph nodes are enlarged and oedematous.

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Figure 6: Clinical case of LSD in Dawa-Chefa District in 2008 (Ethiopia). Circumscribed

nodules on the skin all over the body and swollen superficial lymphnodes (A,B).

Lesions develop on the muzzle, in the nostrils, and in the mouth and pharynx. They show a

ring-like margin where there has been separation from the surrounding healthy epithelium.

Lesions in the larynx and trachea, and throughout the alimentary tract, especially the abomasum,

become ulcerated and necrotic. Mucopurulent nasal discharges, persistent dribbling of infected

saliva, coughing and stertorous (snoring) and often distressed breathing are manifested.

Inflammation and hyperemia of the conjunctiva and cornea of the eyes is common (Davies,

1991; Bowden et al., 2008).

Inflammatory and oedematous swellings of the limbs, brisket and genitalia may develop.

Skin lesions become necrotic. Some remain in situ and others slough leaving a full skin thickness

hole, known as a „sitfast‟, which becomes infected by pus-forming bacteria and can also be

infested by fly strike. Large areas of skin may slough causing substantial down grade of the hide

quality (Green, 1959). Lesions in the skin, subcutaneous tissue, and muscles of the limbs,

together with the severe skin inflammation caused by secondary infection of lesions, greatly

B A

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reduce mobility. Rapid deterioration in body condition results and animals that recover may

remain in poor condition for 1-3 months and in extreme cases for up to 6 months.

Pneumonia is a common and often fatal complication. Absence of oestrus cycles during the

severe debility and abortion is frequent in the early stages due to prolonged fever (Ahmad and

Zaher, 2008). Painful genitalia in bulls can prevent from serving for long periods. Foetus born to

infected cows may show skin lesions at birth presumably acquired through intra-uterine infection

(Davies, 1991).

Pathological lesions

On autopsy, nodules may be found in the subcutaneous tissue, muscle fascia and in muscles,

which are grey-pink with caseous necrotic cores. The subcutis is infiltrated by red watery fluid.

Similar nodules may be scattered through the nasopharynx, trachea, bronchi, lungs, rumen,

abomasum, renal cortex, testicles and uterus (Prozesky and Barnard, 1982).

Histopathological examination shows that the epidermis is extensively necrotic. While in the

intact areas, some ballooning degeneration of squamous epithelial cells with occasional intra-

cytoplasmic inclusions is seen. Prominent lesions of vasculitic necrosis with cell debris and

severe diffuse infiltration with inflammatory cells mainly neutrophils, have been seen in the

superficial and deep dermis (Prozesky and Barnard, 1982). There is a vasculitis and perivascular

infiltration with white cells which causes a thrombosis of the vessels in the dermis and subcutis

(Figure 7). The cells infiltrating the lesion are of a predominantly epithelioid type, which was

described in sheep pox (Burdin, 1959; Davies, 1991; Brenner et al., 2006). There are also

eosinophilic intracytoplasmic inclusions in the epidermal elements of the lesion and the

inflammatory cells. The lesions gradually become necrotic as a result of the thrombosis (Burdin,

1959).

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Figure 7: Vasculitic necrosis with cell debris and severe diffuse infiltration with inflammatory

cells mainly neutrophils, are seen in the superficial and deep dermis; Source: Brenner et al.,

2006.

Diagnosis

LSD can be clinically diagnosed by its pathognomic nodular lesions on the skin, mucous

membranes, swelling of the superficial lymph nodes and systemic involved symptoms by

experienced practitioners. Confirmation of the diagnosis through laboratory techniques can be

done using various methods.

Virus isolation and identification

Rapid confirmation can be made by demonstration of the typical capripox virion in biopsy

material or desiccated crusts using the transmission electro-microscope in combination with the

clinical history of a generalized nodular skin disease and enlarged superficial lymph nodes in

cattle (Figure 8). Capripox is morphologically distinct from Parapox virus which causes bovine

pustular stomatitis and pseudocow pox, but cannot be differentiated from Cowpox and Vaccinia

viruses in Orthopox virus. But neither of these causes a generalized infection and both are

uncommon in cattle (Fenner et al., 1987; Babiuk et al., 2008a; OIE, 2010). LSDV causes a

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characteristic cytopathic effect and intracytoplasmic inclusion bodies, and is distinct from the

virus of pseudo-LSD (Allotron- Herpes mammilitis), which is a herpesvirus producing syncytia

and intranuclear inclusion bodies (Babiuk et al., 2008a).

Figure 8: A Capripox virion from the skin of Capripox infected goat; the virus particle is

indicated by arrow; Source: Babiuk et al., 2008a

Virus isolation could be attempted and is best carried out in primary lamb kidney cell or lamb

testis cell cultures. Secondary lamb testis cell line (OA3.Ts) has been proved to replace the

primary cell cultures for better efficiency and easily managed to grow Capripoxvirus (Babiuk et

al., 2007). LSDV can be grown on a variety of sheep, goat and cattle cells (Binepal et al., 2001).

The LSDV isolation can be confirmed by Immunostaining technique using anti- Capripoxvirus

serum which allows the visualization of the LSDV plaques in the cell culture (Babiuk et al.,

2007). Antigen detection can be demonstrated in tissue culture using immunoperoxidase or

immunofluorescent staining (OIE, 2010). A Polymerase Chain Reaction (PCR) technique to

detect capripoxvirus antigen from cell culture and biopsy specimens has been developed and the

reagents are available commercially (Irland and Binepal, 1998; Heine et al., 1999; Tuppurainen

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et al., 2005; Bowden et al., 2008). An immunocapture Enzyme-linked immunosorbent assay

(ELISA) for the detection of Capripoxvirus antigen is also reported (Rao et al., 1997).

Serodiagnosis

Neutralizing antibody appears 3-4 days after the onset of the clinical signs and reaches the peak

titre level in 2-3 weeks. Both complement fixing and precipitating antibodies are present in the

serum of infected and recovered animals. Immunological defense against capripoxvirus relies

mainly on cell-mediated immune response and humoral immunity would remain in the

circulation for a short period within the time range of mostly seven to eight months (Capstick

and Coackley, 1962; Lefèvre and Gourreau, 2010; OIE, 2010).

Virus Neutralization Test (VNT): VNT is the most common widely used serological test for

capripox antibody detection (Davies and Otema, 1981; Babiuk et al., 2008a; OIE, 2010). It has

high specificity to rule-out false positives due to cross- reaction with cowpox and Parapoxvirus

antibodies but its sensitivity is lower to trace small antibody titration (Davies and Otema, 1981).

Indirect Fluorescence Antibody Test (IFAT): An indirect test using the capripoxvirus antigen

fixed in the tissue culture plate can be used to detect antibodies against LSD in the serum. The

test was reported to have good sensitivity but cross reacting Parapox and Orthopox viruses might

affect its specificity at lower serum dilution rates (Davies and Otema, 1981).

Western blotting assay is a specific and sensitive test, however, it is difficult to perform and

interpret (Chand et al., 1994). An antibody ELISA based on P32 recombinant antigen was

developed and the preliminary test evaluation done but it is not yet validated to replace the

conventional tests (Heine et al., 1999). Indirect ELISA based on inactivated whole antigen from

sheeppoxvirus was reported to detect capripox antibody in experimentally infected animals

(Babiuk et al., 2009). Recombinant CPV Antigen ELISA was also reported to detect serum

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antibody from experimental infected sheep and goats which is still under development (Bowden

et al., 2009). Agar gel Immunodiffusion test (AGID) has been used for detecting the

precipitating antigen of capripoxvirus, but has the disadvantage that this antigen is shared with

Parapoxvirus and has also less sensitivity (OIE, 2010). So far a diagnostic assay that can be

easily run for an epidemiological study of LSD is not yet validated and commercially not

available. Moreover, the accuracy of the conventional diagnostic techniques which are currently

being used for diagnosis purposes have not been evaluated in particular in the context of the

target population in Ethiopia.

Differential diagnosis

Skin diseases of cattle that could be considered as differential diagnosis are:

Bovine Herpes Mammilitis (Pseudo-lumpy skin disease): The presence of Bovine Herpes

Mammilitis case has not yet been confirmed by laboratory in Ethiopia.

Dermatophilosis: Dermatophilus congolensis infection is one of wide spread skin disease of

cattle in Ethiopia and lesions could be differentiated from LSD in that the lesions of

dermatophilosis are superficial (often moist and appear as crusts of keratinized material) scabs of

0.5- to 2 cm diameter. The organism can be demonstrated by Giemsa staining.

Demodicosis, Besnoitiosis, Photosensitization, insect bites; and Ringworm could also be

considered as the differential diagnosis. But epidemiological features could help to distinguish

LSD vs. other skin lesions.

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5. Control and Prevention

Control and prevention of LSD in endemic countries like Ethiopia relies mainly on vaccination.

The experience in the major parts of the country showed that the vaccination approach is

commonly chosen and is often that of ring vaccination around a local foci outbreak when it

occurs. Animals that recover from virulent LSD infection generate lifelong immunity consisting

both of a humoral and cell mediated protective immunity (Kitching et al., 1987). Maternal

immunity provides protection from LSD in calves at least for 6 months (Davies 1991). In South

Africa, the control of insects was not effective in preventing the spread of LSD, but current

insecticides together with repellents might help to reduce the spread of LSD (Davies, 1991).

There is no specific treatment for LSD, but early stage antibiotic treatment could reduce

secondary bacterial complications to improve recovery process.

Vaccines for LSD control

Attenuated vaccines of different capripoxvirus strain origins are available to protect cattle, sheep

and goats. LSD (Neethling strain), Kenya SGPV, Romanian sheep pox and Gorgon goat pox

(from Iraq) have all been shown to be serologically identical by fluorescent antibody and serum

VNT (Davies and Otema, 1981). Therefore, it is likely that many of these vaccine strains

available in different parts of the world would be suitable for the prophylaxis of LSD (Kitching

et al., 1987; Davies, 1991; Kitching, 2003). These live attenuated vaccines are mainly

stimulating the cell mediated immune response.

Two different vaccines have been widely used for the control of LSD in cattle populations in

Africa. In southern Africa, the Neethling strain was passaged 50 times in tissue cultures of lamb

kidney cells and then 20 times in embryonated eggs (OIE, 2010). The strain proved to be

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innocuous and immunogenic for cattle, although local reactions do occur in a high proportion of

animals at the vaccination site. No generalization of infection has ever followed its use. It is

produced in tissue culture and issued as a freeze-dried product (Capstick and Coackley, 1961a;

Weiss, 1968).

In Kenya, an effective vaccine has been produced from a local strain of sheep and goat pox virus

(SGPV). The SGPV was passaged 18 times in pre-pubertal lamb testes or foetal muscle cell

cultures and used for vaccination at this level (OIE, 2010). This was shown to immunize cattle

against LSD (Capstick and Coackley, 1961a; Carn, 1993). Local reactions have not been seen,

but some Bos taurus breeds have shown lymphadenitis with signs of mild, generalized LSD-like

lesions following vaccination (approximately 0.02%) (Yeruham et al., 1994). These reactions

were not reproduced in the laboratory, and no such reactions have ever been observed in Bos

indicus cattle. In Ethiopia both Kenyan SGPV and Neethling strain vaccines are produced at the

National Veterinary Institute (NVI) and the Kenyan SGPV strain is widely used for all cattle,

sheep and goats.

Two other strains of sheep pox vaccine have recently been used as a prophylaxis against LSD.

The Romanian strain, prepared in the skin of lambs for use against sheep pox, was used in

several million cattle in Egypt and appeared to be immunogenic (Michael et al., 1996). Another

sheep pox strain, the RM 65 prepared in tissue culture, was used in Israel. No complications have

followed the use of these strains in cattle. However, re-infection of the beef cattle has been

reported in Israel during 2006/07 epidemics after vaccination with the RM65 sheeppox vaccine

(Brenner et al., 2009). In general problems related to vaccine failure and re-infection of

vaccinated animals have been getting higher magnitude which should draw the attention of

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researchers and vaccine production institutes to envisage for better immunogenic CPV vaccines

in the future.

Studies with both the Neethling and the Kenya SGPV strains showed that an immunizing dose of

103.5 TCID50 is desirable for field vaccination campaigns. It is suggested that 10 to 50 times the

sheep immunizing dose should be used for cattle to protect from LSD (Davies, 1991). After a

single inoculation, solid immunity lasts for at least 3 years and probably longer (Capstick and

Coackley, 1961a).

Serological studies with vaccinated cattle have shown that many animals resist challenge with

virulent LSDV when they have no detectable fluorescent or neutralizing antibody to the virus.

Most animals do show a serological response after field infections with wild LSDV, however,

the vaccinal strain does not elicit detectable humoral immunity (Babiuk et al., 2008b). There is

an important cellular component of the immune response to LSD in cattle, as there is to other

pox viruses and based on this principle Capstick and Coackley (1962) developed a

hypersensitivity test to determine the susceptibility of cattle to LSD for use in vaccination studies

(Capstick and Coackley, 1962). This test can be used to determine the responses to vaccination.

New Recombinant Vaccines

The large size genome and resistance to heat characteristics of LSDV has render this virus a very

useful and efficient vector of expression for the construction of recombinant vaccines (Lefèvre

and Gourreau, 2010). A new generation of capripox vaccines are being developed that use

capripoxvirus genome as a vector for the genes of other ruminant pathogens, for instance genes

of Rinderpest (Romero et al., 1993), PPR (Diallo et al., 2002; Berhe et al., 2003), Rabies

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(Aspden et al., 2002) and RVF (Wallace and Viljoen, 2005; Wallace et al., 2006). These

prospective recombinant vaccines under development could provide protection against LSD and

the counterpart diseases inserted as recombinant antigen in a single dose vaccination (Babiuk,

2002).

Control and Eradication in Disease free countries

If LSD is confirmed in a new area before extensive spread occurs, the area should be

quarantined, the infected and in contact animals slaughtered, and the premises cleaned and

disinfected as an attempt to eradicate the disease from the country (Davies, 1991; Babiuk et al.,

2008a; OIE, 2010). Ring vaccination of cattle within the quarantine in the radius of 25-50km and

strict animal movement controls should be considered (Yeruham et al., 1995).

If the disease has spread over a large area, the most effective means of controlling losses from

LSD is mass vaccination. However, even with vaccination, consideration should still be given to

eliminating infected and exposed herds by slaughter, proper disposal of animals and

contaminated material, and by cleaning and disinfecting contaminated premises, equipment, and

facilities (Anon., 2008, 2010).

6. Economic Importance

Economic losses due to LSD depend on the magnitude of production losses due to morbidity and

mortality. Milk yield fall more than 50% in affected herds has been reported and concurrent

purulent mastitis which can cause loss of quarters could accentuate the fall in milk production

(Woods, 1988; Lefèvre and Gourreau, 2010). The full skin thickness lesions of LSD punch holes

right through the hide, thereby causing permanent damage (Green, 1959; Prozesky and Barnard,

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1982). Secondary infections of the skin and lung lesions results in further debility often causes

culling. Abortion may occur due to prolonged fever lasting up to 72 hours and it is not

uncommon in the early stages. Temporarily sterility in bulls and extended delay in coming to

estrous in cows due to debility had been recorded. Economic losses to the producers in terms of

physical loss impact could be comparable to that caused by FMD (Babiuk et al., 2008a).

High susceptibility of high producing breeds imported from Europe or Australia could also pose

a considerable hindrance for the development of small scale and intensive dairy production in

Africa and in particular in Ethiopia. Being one of the transboundary diseases, Capripox viruses

could have impediments to livestock and livestock product trades. This could affect particularly

the economic well-being of the farmers and that of pastoral communities but also more globally

the country‟s economy (Rich and Perry, 2010).

Capripoxviruses in general have a single serotype, have no carrier state, have a limited host

range and vaccines are available that provide long lasting immunity. These attributes increase the

prospect of successful implementation of regional control programs, leading to the elimination of

the virus and conceivably eradication (Babiuk et al., 2008a).

7. The Objective and goals of the PhD research

General objective

The general objective of this thesis is to gain understanding in the epidemiology and

economic impact of LSD in Ethiopia in order to propose practical control and prevention

options.

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Specific objectives

Three specific objectives were envisaged and each would be treated in a separate chapter

(Chapter 2, 3 and 4) in the subsequent compilation of the thesis.

1. To determine the diagnostic and screening tests that could be used for Epidemiological

study of LSD: Lack of information on the performance and characteristic of the

diagnostic tests could substantially affect the result and parameter estimates of

epidemiological studies and it should be considered as a precursor step. The accuracy of

diagnostic tests should also be evaluated for specific target population in Ethiopia. There

is no perfect (gold standard) test for the diagnosis of LSD and thus the novel Bayesian

approaches to evaluate the performance of diagnostic tests without gold standard was

chosen to estimate the accuracy of Indirect Fluorescent Antibody test (IFAT) and Virus

neutralization test (VNT) under field study in Ethiopia (Gari et al., 2008).

2. To estimate the prevalence at herd-level and animal-level and to identify and quantify the

risk factors associated with the disease occurrence in Ethiopia:

An epidemiological study which encompassed the different agro-ecological zones of

Ethiopia was undertaken. Serological sampling was concurrently conducted with the risk

factor study to estimate the sero-prevalence and to analyze the risk factors associated

with LSD occurrences. The risk factor analysis in this study has enlightened the

important risk factors associated with LSD occurrence for the future targeted measures to

control and prevent the disease (Gari et al., 2010). Because of time limitation the

serological test results could not be included in the thesis. The control opinions proposed

should be complemented with economic feasibility investigations for the cost-effective

way of controlling the disease at different scales and scenario to provide guidance for

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eventual knowledge-based decision process for the control of the disease or to mitigate

the risks to an acceptable level. The next study emphasizes on the financial cost-benefit

of LSD control.

3. Evaluation of financial cost of clinical LSD at the farm level and the economic benefit of

its control by vaccination:

The farm level financial impact and the economic benefit of LSD control by annual

vaccination were evaluated in selected districts in Ethiopia. The result of this study would

provide an insight for the producers and the government to endeavour the control of the

disease. Manuscript is prepared from this work and it is submitted to Prev. Vet. Med.

Journal.

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Chapter II.

Article 1 : Evaluation of indirect fluorescent antibody test (IFAT) for the

diagnosis and screening of lumpy skin disease using Bayesian method

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Evaluation du test d’immunofluorescence indirect (IFI) pour le diagnostic et

le dépistage de la Dermatose Nodulaire Contagieuse Bovine à l’aide de

méthodes bayésiennes.

G. Gari, F. Biteau-Coroller, C. LeGoff, P. Caufour, F. Roger

Résumé :

Deux tests de diagnostic sérologique de la Dermatose Nodulaire Contagieuse (DNC) Bovine ont

été développés, l‟immunofluorescence indirecte (IFI) et la séroneutralisation virale (SNV) mais

aucun des deux n‟a encore été évalué dans le contexte éthiopien. C‟est pourquoi, ce travail a eu

pour objectif d‟évaluer les performances de l‟IFI pour le diagnostic et le dépistage de masse de la

DNC en Ethiopie en utilisant le test SNV en comparaison. Une méthode bayésienne a été utilisée

dans la mesure où il n‟existe pas de gold standard. Des prélèvements de sang ont été effectués

dans deux sous populations de bovins, la première dans le nord où des épizooties récentes de

DNC ont été observées récemment et la seconde dans le sud du pays. Cette étude a permis de

montrer que la spécificité (88%) et la sensibilité (92%) de l‟IFI étaient bonnes et que le test

pouvait être utilisé dans des dépistages sérologiques avec un relativement faible nombre de faux

positifs ou négatifs. Le test SNV, très spécifique mais moins sensible, devrait être réservé à la

confirmation de cas douteux.

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Evaluation of indirect fluorescent antibody test (IFAT) for the diagnosis and

screening of lumpy skin disease using Bayesian method

G. Gari, F. Biteau-Coroller, C. LeGoff, P. Caufour, F. Roger

Summary

Diagnostic tests are important tools for epidemiological studies in that they enable to detect and

quantify non-clinical disease events in the population. Interpretation of sero-diagnostic data is

conditioned by the sensitivity and specificity of the tests used and the prevalence of the disease

in the study area. One needs to know the accuracy of the diagnostic test to adjust for the

misclassification of the

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