Impact des mycorhizes sur la revégétation et la succession végétale primaire dans un...

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Impact des mycorhizes sur la revégétation et la succession végétale primaire dans un écosysystème hostile Martina Janoušková, Institute of Botany ASCR, Czech Republic Dr. Diederik van Tuinen, UMR INRA-1088; CNRS-5184; U. de Bourgogne INRA-CMSE, Plant-Microbe- Environment Dijon France

Transcript of Impact des mycorhizes sur la revégétation et la succession végétale primaire dans un...

Impact des mycorhizes sur la revégétation et la succession végétale primaire dans un écosysystème hostile

Martina Janoušková, Institute of Botany ASCR, Czech Republic

Dr. Diederik van Tuinen, UMR INRA-1088; CNRS-5184;U. de BourgogneINRA-CMSE, Plant-Microbe-EnvironmentDijon France

PLANTSPLANTSTripleurospermum inodorumTripleurospermum inodorumCalamagrostis epigejosCalamagrostis epigejosSisymbrium loeselii Sisymbrium loeselii

AM FUNGIAM FUNGIG. intraradices G. intraradices BEG140BEG140G. intraradices G. intraradices BEG141BEG141G. claroideum G. claroideum BEG96BEG96

Experimental designExperimental designExperimental designExperimental design

PLANTSPLANTSTripleurospermum inodorumTripleurospermum inodorumCalamagrostis epigejosCalamagrostis epigejos

AM FUNGIAM FUNGINon-inoculatedNon-inoculatedG. intraradices G. intraradices BEG140BEG140G. claroideum G. claroideum BEG96BEG96G. intraradices G. intraradices BEG140 + BEG140 + G. claroideum G. claroideum BEG96BEG96

10 replicates10 replicatesHarvest at 4 and 8 weeks after Harvest at 4 and 8 weeks after inoculationinoculation

ANALYSESANALYSESFresh and dry weightFresh and dry weightLevel of colonisation by AM fungi (Trypan blue)Level of colonisation by AM fungi (Trypan blue)Alkaline phosphatase activityAlkaline phosphatase activityIntensity of root colonisation by each fungus (PCR)Intensity of root colonisation by each fungus (PCR)Expression of selected fungal genes ofExpression of selected fungal genes ofG. intraradices G. intraradices (qPCR)(qPCR)

Why collaborate?Why collaborate?

• Working on degraded experimental sites.

• Big collection of AM fungi isolated from degraded environments.

• Developed molecular tools to monitor fungal gene expression in plant roots, and to detect the fungus in the roots.

Results and futureResults and future

• Use of the molecular tools to monitor and assess the fungal fitness in field conditions

• Selection of new markers appropriated for fungal efficiency monitoring

Glomus intraradicesGlomus intraradices genome sequencing project genome sequencing projectGlomus intraradicesGlomus intraradices genome sequencing project genome sequencing project