Appel d'offres 'Méthodes d'analyse des denrées alimentaires...

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Appel d'offres "MÉTHODES D’ANALYSE DES DENRÉES ALIMENTAIRES AU REGARD DE L’HYGIÈNE ALIMENTAIRE" (Mandat de normalisation M/381 adressé au CEN) Le 12 février 2007 La Commission européenne charge le CEN d’établir des méthodes de référence normalisées et validées dans le cadre de la législation communautaire dans le domaine de l’hygiène alimentaire, y compris de l’environnement de production. Les méthodes validées normalisées sont en effet d'importance majeure pour assurer une application et un contrôle uniformes de la législation européenne dans tous les États membres. Trois types de méthodes d'analyse sont demandés : 1) Élaboration de normes CEN validées ; 2) Élaboration de normes CEN validées à partir de normes ISO existantes ; 3) Validation des normes CEN existantes et révision de normes. Créé en 1961, le CEN (Comité Européen de Normalisation) est un système décentralisé comprenant le réseau de ses 30 membres nationaux et le Centre de Management du CEN (CMC). La mission du CEN est d'œuvrer en faveur de l'économie européenne, en particulier dans le cadre des échanges internationaux, du bien-être des citoyens européens et de l'environnement, en offrant aux parties intéressées une structure efficace pour l'élaboration, la mise à jour et la diffusion d'ensembles cohérents de normes et spécifications. AFNOR représente la normalisation française dans les réseaux de la normalisation européens et internationaux. AFNOR détient le secrétariat du CEN/TC 275/WG 6 (le TC 275 est le comité technique dédié aux méthodes d'analyse horizontales en agro-alimentaire et le WG 6 est le groupe de travail consacré à la microbiologie des aliments, y compris l'environnement de production). Le travail mandaté sera effectué au sein du CEN/TC 275/WG 6. AFNOR s'occupera de la gestion administrative des travaux de normalisation. Vous trouverez ci-après un appel d'offre pour recherche de chefs de projet. Ce document explique la portée du mandat, la liste des méthodes demandées et les modalités de réponses à l'appel d'offre. Les parties intéressées sont priées de contacter l'AFNOR : Sandrine Espeillac ([email protected] ), secrétariat du CEN/TC 275/WG 6, aussitôt que possible, dans tous les cas avant le 18 mars 2007. Contact AFNOR Sandrine Espeillac 11 rue Francis de Pressensé F-93571 La Plaine Saint-Denis Cedex Tel.: +33 1 41 62 86 02 Fax: +33 1 49 17 90 00 E-mail: [email protected] Web: www.afnor.org 1

Transcript of Appel d'offres 'Méthodes d'analyse des denrées alimentaires...

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Appel d'offres "MÉTHODES D’ANALYSE DES DENRÉES ALIMENTAIRES AU REGARD DE L’HYGIÈNE ALIMENTAIRE" (Mandat de normalisation M/381 adressé au CEN) Le 12 février 2007 La Commission européenne charge le CEN d’établir des méthodes de référence normalisées et validées dans le cadre de la législation communautaire dans le domaine de l’hygiène alimentaire, y compris de l’environnement de production. Les méthodes validées normalisées sont en effet d'importance majeure pour assurer une application et un contrôle uniformes de la législation européenne dans tous les États membres. Trois types de méthodes d'analyse sont demandés : 1) Élaboration de normes CEN validées ; 2) Élaboration de normes CEN validées à partir de normes ISO existantes ; 3) Validation des normes CEN existantes et révision de normes.

Créé en 1961, le CEN (Comité Européen de Normalisation) est un système décentralisé comprenant le réseau de ses 30 membres nationaux et le Centre de Management du CEN (CMC). La mission du CEN est d'œuvrer en faveur de l'économie européenne, en particulier dans le cadre des échanges internationaux, du bien-être des citoyens européens et de l'environnement, en offrant aux parties intéressées une structure efficace pour l'élaboration, la mise à jour et la diffusion d'ensembles cohérents de normes et spécifications.

AFNOR représente la normalisation française dans les réseaux de la normalisation européens et internationaux. AFNOR détient le secrétariat du CEN/TC 275/WG 6 (le TC 275 est le comité technique dédié aux méthodes d'analyse horizontales en agro-alimentaire et le WG 6 est le groupe de travail consacré à la microbiologie des aliments, y compris l'environnement de production).

Le travail mandaté sera effectué au sein du CEN/TC 275/WG 6. AFNOR s'occupera de la gestion administrative des travaux de normalisation. Vous trouverez ci-après un appel d'offre pour recherche de chefs de projet. Ce document explique la portée du mandat, la liste des méthodes demandées et les modalités de réponses à l'appel d'offre. Les parties intéressées sont priées de contacter l'AFNOR : Sandrine Espeillac ([email protected]), secrétariat du CEN/TC 275/WG 6, aussitôt que possible, dans tous les cas avant le 18 mars 2007. Contact AFNOR Sandrine Espeillac 11 rue Francis de Pressensé F-93571 La Plaine Saint-Denis Cedex Tel.: +33 1 41 62 86 02 Fax: +33 1 49 17 90 00 E-mail: [email protected] Web: www.afnor.org

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Appel d'offre "Chefs de projet" I- PRESENTATION 1. Portée et contexte réglementaire Le mandat M/381 est un mandat de normalisation adressé au CEN par la Commission européenne, dans le contexte de la législation sur l’hygiène alimentaire. Ce mandat s’inscrit dans les dispositions assurant la sécurité alimentaire au regard des risques biologiques. Il s’agit notamment : - du règlement (CE) n° 882/2004 du Parlement européen et du Conseil du 29 avril 2004 relatif aux contrôles officiels effectués pour s'assurer de la conformité avec la législation sur les aliments pour animaux et les denrées alimentaires et avec les dispositions relatives à la santé animale et au bien-être des animaux - du règlement (CE) n° 852/2004 du Parlement européen et du Conseil du 29 avril 2004 relatif à l’hygiène des denrées alimentaires - du règlement (CE) n° 853/2004 du Parlement européen et du Conseil du 29 avril 2004 fixant des règles spécifiques d’hygiène applicables aux denrées alimentaires d’origine animale - du règlement (CE) n° 854/2004 du Parlement européen et du Conseil du 29 avril 2004 fixant les règles spécifiques d’organisation des contrôles officiels concernant les produits d’origine animale destinés à la consommation humaine - de la directive 2003/99/CE du Parlement européen et du Conseil du 17 novembre 2003 sur la surveillance des zoonoses et des agents zoonotiques, modifiant la décision 90/424/CEE du Conseil et abrogeant la directive 92/117/CEE du Conseil - du règlement (CE) n° 2073/2005 sur les critères microbiologiques applicables aux denrées alimentaires. L'un des objectifs du mandat est d'élaborer des méthodes normalisées validées en soutien de la politique de l'Union européenne en hygiène alimentaire.

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2. Liste des sujets mandatés La Commission demande au CEN de produire les méthodes normalisées ci-dessous et de garantir que ces méthodes ont été pleinement validées par des études préliminaires (validation intralaboratoire) et des études collaboratives (validation interlaboratoires). Trois types de méthodes d'analyse sont demandés : 1) Élaboration de normes CEN validées

Projet Detection of food-borne viruses in food based on PCR technique (Norovirus genogroup I, Norovirus genogroup II, Hepatitis A) Detection of Histamine Detection of Staphylococcal enterotoxin in dairy products and other matrices Detection of Bacillus cereus enterotoxin detection in food (emetic and diarrheal toxins) Detection of Enterobacter sakazakii 2) Élaboration de normes CEN validées à partir de normes ISO existantes

Projet Document de référence Detection of pathogenic Vibrio: Detection of Vibrio parahaemolyticus and Vibrio cholerae

ISO/TS 21872 –1

Detection of pathogenic Vibrio: Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae

ISO/TS 21872 –2

Detection and enumeration of Enterobacteriaceae by MPM technique with pre-enrichment

ISO 21528-1

Enumeration of Campylobacter ISO TS 10272-2 3) Validation des normes CEN existantes et révision de normes

Projet Document de référence Detection of Listeria monocytogenes: detection

EN/ISO 11290 –1 /Amdt1

Enumeration of Listeria monocytogenes: enumeration

EN/ISO 11290 –2 /Amdt1

Detection of E. coli O157 EN/ISO 16654 Detection of Campylobacter EN/ISO 10272 –1 Detection of Salmonella in faeces EN/ISO 6579/Amd. 1 Detection of Presumptive Yersinia enterocolitica

EN/ISO 10273

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II- OFFRE 1. Description - Un chef de projet par sujet sera choisi - Les chefs de projet seront sous-traitants d'AFNOR - Un chef de projet peut candidater pour plusieurs projets - Les réunions seront organisées à Paris ou Bruxelles ou à d'autres endroits faciles d'accès - Les projets du tableau 1 doivent être achevés et publiés au 31-12-2012, les autres au 31-12-2011. • Les principales tâches des chefs de projet seront (voir aussi les tâches spécifiques

par projet dans l'annexe): - Discussion des résultats des travaux au sein du groupe de travail correspondant - Préparation du projet de norme - Evaluation des commentaires techniques soulevés durant les réunions et durant la phase d'enquête du projet de norme - Mise à jour du projet - Organisation des réunions du groupe de travail - Organisation des études collaboratives - Le chef de projet sera responsable de la gestion de son projet et devra informer le CEN/TC 275/WG 6 du suivi du projet - Le chef de projet devra respecter les dates limites des délivrables (en cas de retard, des pénalités financières seront appliquées). • Aide financière - Il y aura une aide financière de la Commission européenne et de l'AELE pour la partie validation - Les coûts des chefs de projet et des sous-traitants devront être justifiés avec des copies des factures appropriées. Toutes les preuves appropriées devront être conservées en vue des futurs paiements (rapports d'essais, travaux, les projets de normes, les délivrables, fiches de présence, contrats et factures, billets, cartes d'embarquement, factures d'hôtel, listes de présence signées, ordres du jour des réunions et rapports des réunions, factures (matériel, etc.), bons d'achat etc.....) - L'aide financière de la Commission européenne et de l'AELE est basée sur Framework Partnership Agreement (FPA). Les sous-traitants devront satisfaire aux règles du FPA (responsabilité, propriété des résultats, confidentialité…) - Les coûts encourus avant la signature du contrat ne seront pas couverts. Pour de plus amples informations, merci de contacter Sandrine Espeillac. 2. Critères d'éligibilité Sera en situation d'exclusion le candidat qui: - a fait l'objet d'une condamnation non susceptible de recours pour une infraction professionnelle ; - est en situation fiscale ou parafiscale irrégulière ; - fournit des renseignements incomplets ou erronés

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3. Critères de sélection des chefs de projets

• Connaissance et expérience technique de la normalisation européenne et internationale

• Expertise scientifique et technique sur le projet mandaté et sur la méthode

d'analyse étudiée • Expérience dans l'organisation des études collaboratives selon les protocoles de

normalisation conformément au règlement CE n° 2073/2005 • Expérience de la gestion de projet et de groupes de travail • Capacité à fournir les délivrables aux dates indiquées.

4. Procédure de candidature Les parties intéressées sont priées de contacter l'AFNOR : Sandrine Espeillac ([email protected]), secrétariat du CEN/TC 275/WG 6, aussitôt que possible, dans tous les cas avant le18 mars 2007. Les candidatures doivent être rédigées en anglais. Les candidatures devront contenir: - a Curriculum Vitae synthétique, démontrant les expertises requises - une décomposition des tâches, des coûts estimés et des frais de déplacements - un planning de la réalisation des tâches - une déclaration signée, par laquelle le candidat certifie ne pas être dans l'une des situations décrites au point 2, certifie aussi que tous les documents joints sont véridiques et conformes à la réalité. La sélection et la nomination du chef de projet seront conduites par Sandrine Espeillac (AFNOR, secrétariat du CEN/TC 275/WG 6) et Alexandre Leclercq (Animateur du CEN/TC 276/WG 6).

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ANNEXE : Détail des projets 1. Detection of food-borne viruses in food based on PCR technique

• Relation to the mandate M 381 This project shall cover item 1, table 1 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275167 included in the work program of CEN/TC 275 as preliminary work item.

• Title Microbiology of food and animal feeding stuffs – Detection of food-borne viruses in food.

• Scope This standard describes the detection of food-borne viruses in food based on PCR technique: - Norovirus genogroup I - Norovirus genogroup II - Hepatitis A

• Starting document No available standardized method yet Documents discussed within CEN/TC 275/WG 6/TAG 4 "Virus"

• Tasks project leader - Discuss the results of the work within CEN/TC 275/WG 6/TAG 4 "Virus" - Preparation of the draft standard - Evaluation of technical comments given during meetings and during enquiry stage of the draft standard. - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated Technical Specification CEN/TS xxxx “Microbiology of food and animal feeding stuffs – Detection of food-borne viruses in food” (and a European Standard EN xxxx in a second step)

• Target dates (provided the financial mandate is approved at the end of December) Final publication of the TS in December 2012

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2. Detection and quantification of histamine

• Relation to the mandate M 381 This project shall cover item 2, table 1 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275168 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs – Detection of histamine.

• Scope This standard describes the detection of histamine in food.

• Starting document No available standardized method yet

• Tasks project leader - Create a new working group within CEN/TC 275/WG 6 on histamine - Discuss the results of the work within this new working group - Preparation of the draft standard - Evaluation of technical comments given during meetings and during enquiry stage of the draft standard. - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated Technical Specification CEN/TS xxxx “Microbiology of food and animal feeding stuffs – Detection of histamine in food” (and a European Standard EN xxxx in a second step)

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication of the TS in December 2012

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3. Detection of staphylococcal enterotoxins in dairy products and other matrixes

• Relation to the mandate M 381 This project shall cover item 3, table 1 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275169 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs – Detection of staphylococcal enterotoxins in dairy products and other matrixes.

• Scope This standard describes the detection of histamine of staphylococcal enterotoxins in dairy products and other matrixes. The method will contain 2 steps: an extraction step and the detection step.

• Starting document No available standardized method yet

• Tasks project leader - Create a new working group within CEN/TC 275/WG 6 on Staphylococcal enterotoxin - Discuss the results of the work within this new working group - Preparation of the draft standard - Evaluation of technical comments given during meetings and during enquiry stage of the draft standard. - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated Technical Specification CEN/TS xxxx “Microbiology of food and animal feeding stuffs – Detection of Detection of staphylococcal enterotoxins in dairy products and other matrixes” (and a European Standard EN xxxx in a second step).

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication of the TS in December 2012

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4. Detection of Bacillus cereus enterotoxins in food (emetic and diarrheal toxins)

• Relation to the mandate M 381 This project shall cover item 4, table 1 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275170 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs – Detection of Bacillus cereus enterotoxins in food (emetic and diarrheal toxins)

• Scope This standard describes the Detection of Bacillus cereus enterotoxins in food (emetic and diarrheal toxins).

• Starting document No available standardized method yet

• Tasks project leader - Create a new working group within CEN/TC 275/WG 6 on Bacillus cereus enterotoxins - Discuss the results of the work within this new working group - Preparation of the draft standard - Evaluation of technical comments given during meetings and during enquiry stage of the draft standard. - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated Technical Specification CEN/TS xxxx “Microbiology of food and animal feeding stuffs – Detection of Bacillus cereus enterotoxins in food (emetic and diarrheal toxins)” (and a European Standard EN xxxx in a second step).

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication of the TS in December 2012

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5. Detection of Enterobacter sakazakii

• Relation to the mandate M 381 This project shall cover item 1, table 2 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275185 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs – Detection of Enterobacter sakazakii

• Scope This standard describes the Detection of Enterobacter sakazakii

• Starting document - ISO/DTS 22964 “Milk and milk products - Detection of Enterobacter sakazakii”. - draft under development within ISO/TC34/SC9 ad’hoc group “Enterobacter sakazakii”

• Tasks project leader - Discuss the results of the work within ISO/TC34/SC9 ad’hoc group “Enterobacter sakazakii” - Preparation of the draft standard - Evaluation of technical comments given during meetings and during enquiry stage of the draft standard. - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated European Standard EN xxxx “Microbiology of food and animal feeding stuffs – Detection of Detection of Enterobacter sakazakii”

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication in December 2012

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6. Horizontal method for the detection of potentially enteropathogenic Vibrio spp. (Detection of Vibrio parahaemolyticus and Vibrio cholerae)

• Relation to the mandate M 381 This project shall cover item 2, table 2 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275186 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs — Horizontal method for the detection of potentially enteropathogenic Vibrio spp. — Part 1: Detection of Vibrio parahaemolyticus and Vibrio cholerae

• Scope This Part 1 of this technical specification specifies a horizontal method for detection of the two main pathogenic Vibrio species causing intestinal illness in humans — V. parahaemolyticus and V. cholerae. Taking into account the remarks made in the introduction, this International Standard is applicable to: - products intended for human consumption and the feeding of animals ; - environmental samples in the area of food production and food handling. WARNING — In order to safeguard the health of laboratory personnel, it is essential that tests for detection of Vibrio, and particularly toxigenic Vibrio cholerae, be conducted only in laboratories equipped for this purpose and under the supervision of an experienced microbiologist, and that great care is exercised in the disposal of contaminated material.

• Starting document - ISO/TS 21872-1 that will be published in 2007

• Tasks project leader - Elaboration of a validated CEN Technical Specification from existing ISO/TS 21872-1. - Discuss the results of the work within ISO/TC 34/SC 9 ad’hoc group “Vibrio” - Revision of the current standard - Evaluation of technical comments given during meetings and during votes. - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated Technical Specification CEN/ISO 21872-1 "Microbiology of food and animal feeding stuffs — Horizontal method for the detection of potentially enteropathogenic Vibrio spp. — Part 1: Detection of Vibrio parahaemolyticus and Vibrio cholerae"

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication of the TS in December 2011

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7. Horizontal method for the detection of potentially enteropathogenic Vibrio spp. (Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae)

• Relation to the mandate M 381 This project shall cover item 2, table 2 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275187 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs — Horizontal method for the detection of potentially enteropathogenic Vibrio spp. — Part 2: Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae

• Scope This Part 2 of this technical specification specifies a horizontal method for detection of the enteropathogenic Vibrio, causing illness in or via the intestinal tract, other than Vibrio parahaemolyticus and Vibrio cholerae. The species detectable by the methods specified in this part include Vibrio fluvialis, Vibrio mimicus and Vibrio vulnificus (for V. vulnificus, see note in 9.4.3). It is not suitable for the isolation of Vibrio hollisae. Strains of V. parahaemolyticus and V. cholerae may also be detected during the application of the methods in this part. Taking into account the remarks made in the introduction, this International Standard is applicable to: - products intended for human consumption and the feeding of animals; - environmental samples in the area of food production and food handling.

WARNING — In order to safeguard the health of laboratory personnel, it is essential that tests for detection of Vibrio, and particularly toxigenic Vibrio cholerae, be conducted only in laboratories equipped for this purpose and under the supervision of an experienced microbiologist, and that great care be exercised in the disposal of contaminated material.

• Starting document - ISO/TS 21872-2 that will be published in 2007

• Tasks project leader - Elaboration of a validated CEN Technical Specification from existing ISO/TS 21872-2. - Discuss the results of the work within ISO/TC 34/SC 9 ad’hoc group “Vibrio” - Revision of the current standard - Evaluation of technical comments given during meetings and during votes. - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated Technical Specification CEN/ISO 21872-2 "Microbiology of food and animal feeding stuffs — Horizontal method for the detection of potentially enteropathogenic Vibrio spp. — Part 2: Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae"

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication of the TS in December 2011

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8. Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae

• Relation to the mandate M 381 This project shall cover item 3, table 2 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275188 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1: detection and enumeration by MPN technique with pre-enrichment

• Scope This International Standard specifies a method for the detection of Enterobacteriaceae with pre-enrichment in products applicable to: - products intended for human consumption and the feeding of animals ; - environmental samples in the area of food production and food handling. Enumeration is carried out by calculation of the Most Probable Number (MPN) after incubation at 37 °C (or 30°C) in liquid medium. This method should be used in both cases: - when the micro-organisms sought are expected to need resuscitation before enrichment ; - when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of the test sample. A limitation on the applicability of this International Standard is imposed by the susceptibility of the methods to a large degree of variability. The methods should be used and the results interpreted in the light of the information given in 11.

• Starting document ISO 21528-1:2004

• Tasks project leader - Elaboration of a validated CEN standard from existing ISO 21528-1. - Discuss the results of the work within ISO/TC 34/SC 9 ad’hoc group “Enterobacteriaceae” - Revision of the current standard - Evaluation of technical comments given during meetings and during votes - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated European Standard EN ISO xxx "Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1: detection and enumeration by MPN technique with pre-enrichment"

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication in December 2011

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9. Detection of Listeria monocytogenes

• Relation to the mandate M 381 This project shall cover item 1, table 3 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275172 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 1: detection method

• Scope This part of ISO 11290 specifies a horizontal method for the detection of Listeria monocytogenes. Subject to the limitations discussed in the introduction, this part of ISO 11290 is applicable to products intended for human consumption or animal feeding.

• Starting document EN ISO 11290-1:1996 and Amendment 1:2004

• Tasks project leader - Validation of the existing EN ISO 11290-1 - Discuss the results of the work within the working group - Revision of the current standard - Evaluation of technical comments given during meetings and during votes - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated EN ISO 11290-1: "Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 1: detection method"

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication in December 2011

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10. Enumeration of Listeria monocytogenes

• Relation to the mandate M 381 This project shall cover item 1, table 3 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275173 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs. Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 2 enumeration method

• Scope This part of ISO 11290 specifies a horizontal method for the enumeration of Listeria monocytogenes. NOTE — The method also allows enumeration of other Listeria species which may be used as indicators of the hygienic quality of food or feed products. Subject to the limitations discussed in the introduction, this part of ISO 11290 is applicable to products intended for human consumption or animal foodstuffs. In general (see note in 9.2.1), the lower limit of enumeration of this method is 10 L. monocytogenes per millilitre of sample for liquid products, or 100 L. monocytogenes per gram of sample for other products.

• Starting document EN ISO 11290-2:1998 and Amendment 1:2004

• Tasks project leader - Validation of the existing EN ISO 11290-2 - Discuss the results of the work within the working group - Revision of the current standard - Evaluation of technical comments given during meetings and during votes - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated EN ISO 11290-2: "Microbiology of food and animal feeding stuffs. Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 2: enumeration method"

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication in December 2011

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11. Detection of Escherichia coli O157

• Relation to the mandate M 381 This project shall cover item 2, table 3 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275171 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli 0157

• Scope This International Standard specifies a horizontal method for the detection of Escherichia coli serogroup O157. Subject to the limitations discussed in the introduction, this International Standard is applicable to products intended for human consumption or for animal feeding stuffs.

• Starting document EN ISO 16654:2001

• Tasks project leader - Validation of the existing EN ISO 16654 - Discuss the results of the work within the working group - Revision of the current standard - Evaluation of technical comments given during meetings and during votes - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data - Validated EN ISO 16654: " Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli 0157"

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication in December 2011

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12. Detection of Campylobacter spp.

• Relation to the mandate M 381 This project shall cover item 3, table 3 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275172 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Campylobacter spp. - Part 1: detection method

• Scope This International Standard describes a horizontal method for the detection of Campylobacter spp. It is applicable to products intended for human consumption or for the feeding of animals and to environmental samples in the area of food production and food handling, subject to the limitations stated in the introduction.

• Starting document EN ISO 10272-1:2006

• Tasks project leader - Validation of the existing EN ISO 10271 - Discuss the results of the work within the working group - Revision of the current standard - Evaluation of technical comments given during meetings and during votes - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data, which will be included in. - Validated EN ISO 10271-1: "Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Campylobacter spp. - Part 1: detection method"

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication in December 2011

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13. Enumeration of Campylobacter spp.

• Relation to the mandate M 381 This project shall cover item 4, table 3 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275173 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: colony-count technique

• Scope This International Standard describes a horizontal method for the enumeration of Campylobacter spp. It is applicable to products intended for human consumption or for the feeding of animals and environmental samples in the area of food production and food handling, subject to the limitations stated in the introduction.

• Starting document ISO/TS 10272-2:2006

• Tasks project leader - Elaboration of a validated CEN Technical Specification from the existing ISO/TS 10272-2. - Discuss the results of the work within the working group - Revision of the current standard - Evaluation of technical comments given during meetings and during votes. - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data, which will be included in. - Validated Technical Specification CEN ISO/TS 10272-2 "Microbiology of food and animal feeding stuffs - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2: colony-count technique"

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication of the TS in December 2011

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14. Detection of Salmonella spp. in primary production stage

• Relation to the mandate M 381 This project shall cover item 5, table 3 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275150 included in the work program of CEN/TC 275 as preliminary work item

• Title ISO 6579/FDAmd 1: Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp. - Amendment 1 Annex D: Detection of Salmonella spp. in animal faeces and in samples from the primary production stage

• Scope This annex is applicable to the detection of Salmonella spp. in: - animal faeces (like poultry, pigs, cattle); - environmental samples in the area of the primary production stage (like dust).

The method in this annex is based upon ISO 6579, with a different selective enrichment. Therefore, where possible reference will be made to the full text of ISO 6579. The selective enrichment medium as described in this annex (being Modified Semi-solid Rappaport Vassiliadis: MSRV) is intended for the detection of motile Salmonellae and is not appropriate for the detection of non-motile Salmonellae.

• Starting document - The future amendment "Amendment 1 Annex D: Detection of Salmonella spp. in animal faeces and in samples from the primary production stage"

• Tasks project leader - Validation of the future amendment "Amendment 1 Annex D: Detection of Salmonella spp. in animal faeces and in samples from the primary production stage" - Discuss the results of the work within the working group - Evaluation of technical comments given during meetings and during votes - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data, which will be included in. - Amendment 1 Annex D of EN ISO 6579: Detection of Salmonella spp. in animal faeces and in samples from the primary production stage

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication in December 2011

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15. Detection of presumptive pathogenic Yersinia enterocolitica

• Relation to the mandate M 381 This project shall cover item 6, table 3 of the mandate.

• WI number CEN/TC 275/WG 6 WI 00275176 included in the work program of CEN/TC 275 as preliminary work item

• Title Microbiology of food and animal feeding stuffs - Horizontal method for the detection of presumptive pathogenic Yersinia enterocolitica

• Scope This International Standard gives general guidance on the detection of Yersinia enterocolitica presumed to be pathogenic to humans. This International Standard is applicable to products for human or animal consumption.

• Starting document - EN ISO 10273:2003

• Tasks project leader - Validation of the existing EN ISO 6579 + amendment - Discuss the results of the work within the working group - Revision of the current standard - Evaluation of technical comments given during meetings and during votes - Update the draft - Organizing the meetings of the working group - Organizing the collaborative studies - The project leader shall manage his project and shall inform CEN/TC 275/WG 6 of the follow up of the project - The project leader shall respect the deadlines of the deliverables (in case of delay, some financial penalties will be applied).

• Deliverables - Report with the validation data, which will be included in. - Validated EN ISO 10273: "Microbiology of food and animal feeding stuffs - Horizontal method for the detection of presumptive pathogenic Yersinia enterocolitica"

• Target dates (provided the financial mandate is approved at the beginning of 2007) Final publication in December 2011