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adsorbed massadsorbed thicknessdensitysurface elastic modulussurface and bulk viscosity

calculatemeasure

Quartz Crystal Microbalance

Excellence in Surface Chemistry

KSV Dissipative QCM-Z500

www.ksvltd.com

Resistance (Dissipation) change

Frequency change

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Biology/Medicine Affinity interactions, e.g.

antibody-antigen Binding of proteins at surfaces

(specific or non-specific) Cell attachment / detachment Cell-membrane specific proteins Deposited lipid bilayers Vesicle attachment

Chemistry Gas/liquid phase measurement Surfactant adsorption Polymer adsorption Nanoparticle adsorption Corrosion Self-assembly Polymeric surface coatings Surface gels

Industry Fouling/cleaning In-line sensing Non-ideal surfaces, e.g. polymers

Adsorption Sensors

Dissipative QCM-Z500

Do you need a simple method to observe the following?

Real-time single- or

multi-channel bench-top

monitoring of adsorption,

binding or fouling at solid

surfaces in liquids or gases!

Vesicle spreading Surface graftingAlternating polyelectrolyte /protein / nanoparticle

adsorption

LB-films, SAMGas sensorsAntigen-antibody bindingReactive modification of

interfaces

The InstrumentThe KSV QCM-Z500 is an acoustic sensor that exploits the highly mass-sensitive resonance vibration of single-crystal quartz to measure the mass of thin surface layers. However, it is much more than a simple adsorptionsensor. By measuring the amount of energy lost (dissipation or damping) at several overtones, the QCM-Z500can provide the viscoelasticity, thickness and density profile of the attached layer.

The Measurement

The QCM substrate, is precoated on one side withthe relevant metal, inorganic or polymeric material.

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The substrate is mounted in the holder, and the instrument assembled. The liquid of interest is introducedvia the tubing system or using a pump. The QCM can also be fitted with a fully automated 4-8 liquid syringedispenser, greatly simplifying repetitive liquid handling tasks.

The measurement is started, and is displayed in real-time.

In Real Time

Plot

Resonance frequency (F or F) Mass change (m) Mass change / unit area (m/A) Dissipation (D or D) Resistance (R or R) Quality factor (Q or Q) Inductance (L or L) Capacitance (C or C).

With Modelling Software

Calculate

Stiffness Elastic modulus Viscous modulus Bulk viscosity Layer thickness Layer density

Obtain adsorbed amount,

layer thickness, density

profiles and viscoelastic

properties of surface layers!

Graphs can be directly copied for reports, or datacan be exported for publication quality presentation.

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Advantages of QCM as an adsorption sensor QCM is an acoustic technique, so there is no requirement for optical clarity

of the liquid phase, or reflectivity of the solid as is the case for optical methodssuch as ellipsometry.

Simple to set up and use The simultaneous measurement of resistance (dissipation) provides crucial

information on the mechanical properties of the material attached to thesurface, e.g. viscoelasticity, thickness and density profile.

Advantages of KSV Instruments as your supplier KSV provides the widest available variety of professionally coated QCM

crystals More than 20 years experience of building instruments for surface chemistry

applications, guarantees you innovative and user-friendly research tools, andrapid, friendly service KSV can efficiently modify instruments to fit special needs, including process

and quality control applications.

Advantages of the KSV QCM-Z500

General The QCM-Z500 can measure on crystals with a wide range of resonance

frequencies User-friendly and intuitive software and instrumentation will have you up and

running quickly Superior measurement speeds, up to hundreds of datapoints/second, allow

observation of high speed processes The excellent temperature stability provides reliable data within minutes,

allowing time dependent studies ranging from seconds to weeks

Impedance analysis By using the widely accepted and proven method of impedance analysis, the

QCM-Z500 provides not only adsorbed mass, but changes in the dissipationof energy at several overtones. The improved amount and quality of raw dataallows for optimal model fitting, required to extract mechanical propertiesof attached layers such as viscous and elastic modulus, density and thickness

Recording of changes in resistance (R), impedance (L), capacitance (C), qualityfactor (Q) and dissipation (D) allow full freedom to further develop theimpedance analysis models

Modular measurement cell and liquid handling options

A quick change of some optional components allows the basic measurementmodule to readily swap between ellipsometric, microscopic and electrochemicalmodes

The parts in contact with the surface and solution can easily be separatedand removed for cleaning and sterilization

A free volume cell can be placed at the interface of a Langmuir trough tostudy interactions between a deposited monolayer and subphase solutes

Flow measurements are optimally performed using the well-defined stagnantpoint flow chamber in combination with a peristaltic pump

Multichannel measurements can be made in up to four separate chamberssimultaneously by hooking together the desired number of instruments, andallows you to place the instruments in different locations when yourmultichannel measurement is finished

The automatic 4-8 syringe multidispenser frees your hands by programmable

introduction of repetitive samples, such as polyelectrolyte multilayers For cases when your liquid sample is scarce, a novel Peltier heating andcooling element has been developed for open surface measurements onminimal liquid volumes placed using an autosampler.

Simultaneous monitoring via

-Microscopy

-Ellipsometry

-Electrochemical cell

Modular cell design and

liquid handling

Open volume cell

Window cell (for microscopy)

Electrochemical attachment

Ellipsometry attachment

L dispensing onto open volume cell

4-liquid multidispenser

Flow cell

Peristaltic pump

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Specifications

Frequency range 0.1 55 MHz

Frequency resolution 0.01 HzMaximum sensitivity in liquid vs. data averaging (5 MHz crystal)Example for 1 datapoint / 6 seconds:Mass resolution, 5 MHz crystal (Sauerbrey) 0.177 ng/cm2

Resistance (R) resolution (Dissipation, D) 0.01 Ohm (1x10-9)*Measurement method True Impedance analysisCrystals Standard 5 MHz, AT-cut

On request 10 MHz, AT-cut

Working Temperature 10 60 oC (active in-cell temperature control)Active sensor area ~ 20 mm2

Maximum mass load ~ 0.1 mg/cm2

Maximum film thickness ~ 10 m (depends on the softness of the material)

Computer Interface EthernetVoltage 100 250 VAC (45-65 Hz)Sample Volume from 300 L (window cell) to 2 mL (Flow cell)

(smaller volumes available on request**)Liquid flow Flow cell stagnant point, batch or continuous (with peristaltic pump)

Other cells laminar flow, batch or continuousMultiliquid autodispenser 4 or 8 liquidsAcquisition speed hundreds of points/second for special applications

Weight & Dimensions

Flow cell 4.75 kg, H 165 mm, W 120 mm, D 210 mmWindow cell 1 kg, H 80 mm, W 100 mm, D 150 mm

Electronics unit 4.5 kg, H 255 mm, W 157 mm, D 250 mm

Shipping Weight 20 kg

Maximum sensitivity in liquid(5 MHz crystal):

1 datapoint / 6 seconds (25 oC) 0.05 Hz, corresponds to 0.9 ng/cm2

1 datapoint / second (25 oC) 0.2 Hz, corresponds to 3.5 ng/cm2

8 datapoints / second (25 oC) 0.5 Hz, corresponds to ~ 9 ng/cm2

* The Resistance (R) resolution corresponds to a Dissipation resolution of about1x10-9. This resolution is several orders of magnitude better than is actually needed, as the change in dissipationnormally observed in a liquid environment lies in the range of a few 10-6.

** Considerably smaller volumes can be used by dispensing onto open volume cell with autosampler.

Further information available online!http://www.ksvltd.com

If you need further information, please visit our website, where you can find background information, examplemeasurements, the full range of accessories and contact details for our distributors. If you dont find whatyou are looking for, please contact us (info@ksvltd.fi).

Online Literature Detailed background information Frequently asked questions Example measurements on

- affinity based systems

- surfactant adsorption- LB film deposition- bulk viscosity measurement- electrochemistry

Active in-cell temperature

control provides

temperature stability, quick

set-up, modularity, flexibility

and open design

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KSV Instruments Ltd.Hylmtie 7, 00380 Helsinki, FINLANDTel. +358-9-5497-3300, Fax +358-9-5497-3333e-mail: info@ksvltd.fi, www.ksvltd.com

KSV Instruments, Inc.P.O.Box 192, Monroe, CT 06468, U.S.A.Toll Free 1-800-280 6216 (U.S.A. only)Tel. +1-203-268 1745, Fax. +1-203-459 0437e-mail: info@ksvinc.com

The CompanyKSV Instruments Ltd is a leading global provider of routine,research and characterization instruments for surface chemistryand Langmuir-Blodgett film applications. Our headquarters arein Helsinki, Finland and we have offices in North America andSweden and trained representatives throughout the world.

InterfacialCharacterizationWith Sigma tensiometers and CAM Drop Shape Analysiscontact angle meters and their various modifications KSVcovers most of the known interfacial measurement techniques.All instruments utilize the latest technological advances inmeasurement of surface/ interfacial tension of liquids as wellas contact angles and surface free energies.

Langmuir FilmPreparation andCharacterizationRanging from manual, educational instruments to fully equippedhigh performance R&D grade multi/alternating layer LB-troughsKSV offers the widest range of Langmuir systems in the world.KSVs BAM (Brewster Angle Microscope) and ISR (InterfacialShear Rheometer) are used for characterization of Langmuirand LB films in a wide variety of nanotechnology, biology and

molecular engineering applications.

Dip CoatingA wide variety of dip-coaters for exacting applications, fromsingle manual dippers to automatic multiple dipping devices.

Process ControlKSV makes a range of at-line (AL) and on-line (OL) processcontrol tools for monitoring surface tension, temperature, pH,viscosity and conductivity. An option also exists for automaticdosing of surfactants.

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